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Original Research Article
In-Vitro antifungal efficacy evaluation of parazone, methylated spirit, and hydrogen peroxide on Cercospora penniseti (Chupp) the causal agent of the millet Cercospora leaf spot
*1Kiyawa, S. A., 2Umar, S., 3Kutama, A.S. and 4Datti, Y.
1Department of Chemistry, Federal College of Education (Technical), Bichi, Kano, Nigeria.
2Department of Biological Sciences, College of Arts, Sciences and Remedial Studies, Kano,Nigeria
3Dept of Biological Sciences, Federal University, Dutse, Jigawa State, Nigeria
4Department of Chemistry, North –West University, Kano, Nigeria
*Corresponding Author Email: ansarinagarr(at)gmail.com
Tel.: +2348065577180
Article Number: irjpeh.003 | Pages: 165-169 | Vol.1 (8),pp. , October 2014 | DOI: http://dx.doi.org/10.15739/irjpeh.003
Received: Accepted: October 6, 2014 Published:
Abstract
The potency of three chemical disinfectants, namely; Parazone, Methylated spirit, and Hydrogen peroxide on Cercospora penniseti was evaluated in-vitro using Phenol Coefficient and Disc Diffusion techniques. Results of the experiment have shown that both techniques were effective for the evaluation of all the three disinfectants.The various disinfectants were found to inhibit the growth of the fungus with significant difference (P>0.05) between the various disinfectants. The phenol coefficients of Hydrogen peroxide, Parazone, Methylated spirit respectively, were found to be 0.9, 1.05 and 1.19. All the disinfectants were found to be active at 5000mm disc concentration against the test organism with inhibition zones ≥ 10 mm diameter indicating that the Phenol Coefficient and Disc Diffusion techniques were efficient for the evaluation of disinfectants’ potency in-vitro and capable of controlling the growth of Cercospora penniseti.
Keywords: Hydrogen peroxide, Parazone, Methylated spirit, Cercospora penniseti, phenol coefficient, disc diffusion techniques
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