International Research Journal of Public and Environmental Health

Original Research Article

Susceptibility of black aspergili conidia to industrial sanitizers


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*Corresponding Author E-mail : lfrison(at)fiq.unl.edu.ar
Tel.: 54-342-4571164 (int. 2541); Fax: 54-342-4571162.

Frison Laura N.

Sobrero Silvina

Fernandez Verónica

Basílico María L.Z


Article Number: irjpeh.022  |   Pages: 65-69  |   Vol.2 (6), June 2015   |   DOI: http://dx.doi.org/10.15739/irjpeh.022

 Received:  Accepted: May 25, 2015  Published:

Abstract

We compared the susceptibility of isolates of black aspergilli (Aspergillus section Nigri) conidia against sodium hypochlorite and peracetic acid. The suspension – neutralization UNE-EN1275 test was performed. Solutions of peracetic acid of 750, 1500, 2250, 3000 and 6000 mg/L and solutions of sodium hypochlorite of 100, 700, 1000 and 1300 mg/L were assessed. Cell suspensions were exposed to sanitizers for 1, 5, 15 and 30 minutes at 20 °C. Mean values of the inactivation kinetic data were fitted to a biphasic inactivation model with the GInaFiT macro software. The time for a 4-log10 reduction in conidia (4Dvalues) for sodium hypochlorite (700, 1000 and 1300 mg/L, pH 6 and 9) ranged from 1.1 to 28.8 minutes and for peracetic acid (6000 mg/L) ranged from 6.2 to 14.3 minutes. The results from this study demonstrate the need to identify contaminating flora because the response of molds pigmented and non-pigmented molds could be different.

Keywords: Black aspergilli (Aspergillus section Nigri), industrial sanitizers, GInaFiT, peracetic acid, sodium hypochlorite, molds pigmented, sanitation processes

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