Original Research Article
Characterization and cytotoxic activity of amygdalin extracted from apricot kernels growing in Egypt
Sherif EA Badr1*, Ola Aly Wahdan2 and Mohamed Saleh AbdelFattah3
1N.M.R. Lab., Regional Center for Food & Feed “RCFF”, Agriculture Research Center “ARC”, Giza, Egypt.
2Regional Center for Food & Feed “RCFF”, Agriculture Research Center “ARC”, Giza, Egypt.
3Natural Products Research Unit, Faculty of Science, Helwan University, Cairo, Egypt.
*Corresponding Author Email: sherif97badr(at)yahoo.com
Sherif EA Badr |
Ola Aly Wahdan |
Saleh AbdelFattah |
Article Number: irjpeh.20.005 | Pages: 37-44 | Vol. 7 (2), March-April 2020 | DOI: https://doi.org/10.15739/irjpeh.20.005
Received: January 15, 2020 Accepted: March 10, 2020 Published: March 21, 2020
Abstract
Vitamin B17 (Amygdalin) is D-mandelonitrile-bi-glucoside naturally found in fruit seeds. Amygdalin was extracted and purified from the kernels of apricots cultivated in Egypt. Identification of the extract was performed by High-performance liquid chromatography (HPLC), where a major amygdalin peak was obtained at retention time 3.5. Chemical structure of amygdalin was verified using Fourier-transform infrared spectroscopy (FTIR) and Nuclear Magnetic Resonance (1H-NMR) spectroscopy. Human hepatic (Huh-7) and colorectal (HT-29) cancer cell lines were treated as indicator for cytotoxicity of extracted amygdalin. The IC50 valued >100 µM and 30 µM, respectively.
Keywords:
Apricot kernels, amygdalin, HPLC, FTIR, NMR, liver and colorectal cancer cell lines, cytotoxicity
How to Cite this Article
Badr SEA, Wahdan OA, AbdelFattah MS (2020).Characterization and cytotoxic activity of amygdalin extracted from apricot kernels growing in Egypt.Int. Res. J. Public Environ. Health. 7 (2):37-44.
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