Original Research Article
Optimization of combination of steam distillation and solvent extraction of Artemisia campestris essential oil using RSM
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*Corresponding Author E-mail : kboutemak(at)yahoo.fr
Khalida Boutemak*1 |
Nasssima Benali2 |
Nadji Moulai-Mostefa3 |
Article Number: irjpeh.17.030 | Pages: 259-269 | Vol.4 (10), November 2017 | DOI: https://doi.org/10.15739/irjpeh.17.030
Received: March 8, 2017 Accepted: April 30, 2017 Published: November 17, 2017
Abstract
Extraction of essential oil (EO) from Artemisia campestris L. leaves using a combination of solvent extraction and steam distillation was investigated. A response surface methodology (RSM), in particular a Box-Behnken design was employed for the optimization of three parameters such as extraction time, liquid-solid ratio and extraction number for the achievement of high yield of extraction by solvent. The optimum extraction conditions were: extraction time of 2h, extraction number equal to 4 and ratio of liquid/solid of 6.5/1 (mL/g). Using these optimal parameters values, the mean obtained value of yield was 26.74 ± 0.11%. EO obtained by the combination of solvent extraction (SE) and steam distillation (SD) yielded 1.3% where the essential oil obtained by only steam distillation (SD) yielded 0.88%. Antibacterial activity showed that essential oil possessed a high activity and significant inhibitory power against Escherichia coli. EO isolated by SE-SD showed reducing ability and exhibited antioxidant activity; it had the best reducing power compared to the essential oil isolated by SD.
Keywords: Artemisia campestris essential oil, extraction, steam distillation, optimization, RSM.
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