International Journal of Agricultural Policy and Research

Original Research Article

Fungi associated with Platypus cylindrus Fab. (Coleoptera: Curculionidae) from Quercus suber L. in North-Eastern Algeria

Mounia AMOURA1*, Maria Lurdes INÀÇIO2, Filomena NÓBREGA2, Luis BONIFACIO2, Edmundo SOUSA2 and Gahdab CHAKALI3


1Université Chadli Bendjedid El-Tarf, Département de Biologie, SNV, Algeria
2Instituto Nacional de Investigação Agrária e Veterinária, I.P., UEISAFSV, Quinta do Marquês, Av. da República,2780-159 Oeiras, Portugal
3Ecole Nationale Supérieure Agronomique, ENSA, Alger, Algeria.

*Corresponding Author Email: sci11vie(at)gmail.com

Mounia AMOURA

Maria Lurdes INÀÇIO

Filomena NÓBREGA

Luis BONIFACIO

Edmundo SOUSA

Gahdab CHAKALI


Article Number: IJAPR.21.001  |   Pages: 1-8  |   Vol. 9 (1), January 2021   |   DOI: https://doi.org/10.15739/IJAPR.21.001

 Received: October 29, 2020  Accepted: November 23, 2020  Published: January 4, 2021

Abstract

The oak pinhole borer, Platypus cylindrus, is an ambrosia beetle capable of establishing symbiotic relationships with many fungi serve, among other things, as a feeding source and/or facilitate tree decline, which is the process of wood tissues decomposition. The aims of this study are to identify the diversity of fungi associated with the oak pinhole borer in cork oak forest of El Kala isolated from galleries, mycangia and intestinal contents of insects. Likewise, this investigation allows to acquire more information on the role of this interaction. A diversity of eleven fungi (Botryosphaeria sp., Penicillium sp., Aspergillus sp., Trichoderma sp., Mucor sp., three Ophiostoma sp., Raffaelea montetyi, Raffaelea canadensis-like and Biscogniauxia mediterranea) belonging to the six systematic orders and originating mainly from Ascomycota were isolated. Molecular characterization was performed based on amplification and sequencing of the D1/D2 domain of large subunit (LSU) ribosomal DNA (rDNA) to confirm the morphological identification of Ophiostoma sp. The sequences obtained revealed 86% similarity with Ophiostoma piliferum and 84% similarity with Ophiostoma sp. In this study, the presence of the species Raffaelea montetyi and Ophiostoma piliferum associated with P. cylindrus confirms its pathogenicity with respect to the health status of the cork oak.

Keywords:

Ambrosia beetle, fungi, ophiostomatales, cork oak, decline.

How to Cite this Article

Amoura M, Inàçio ML, Nóbrega F, Bonifacio L, Sousa E, Chakali G(2021). Fungi associated with Platypus cylindrus Fab. (Coleoptera: Curculionidae) from Quercus suber L. in North-Eastern Algeria. Int. J. Agric. Pol. Res. 9(1):1-8.

Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.


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