International Journal of Agricultural Policy and Research

Original Research Article

Expression of APX1, JcHSP1 and JcHSP2 genes in three Jatropha curcas L. ecotypes under light and water stress in Burkina Faso

Razacswendé Fanta OUEDRAOGO*1, Cyrille BISSEYE2,3, Kouka Fidèle TIENDREBEOGO4, Hemayoro SAMA5, Makido OUEDRAOGO1, Gérard ZOMBRE1 and Jacques SIMPORE3,6.


1Plant Ecophysiology Team, Biosciences Laboratory, Doctoral School of Sciences and Technologies, University Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
2Laboratory of Molecular Biology and Genetics, University Joseph Ki-Zerbo, P.O. Box 7021, Ouagadougou, Burkina Faso.
3Laboratory of Molecular et Cellular Biology (LABMC), University of science and Technology of Masuku (USTM), P.O. Box 943, Franceville, Gabon.
4Genetics and Plant Improvement Team, Biosciences Laboratory, Doctoral School of Sciences and Technologies, Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso.
5Laboratory of Biochemistry and Applied Chemistry (LABIOCA), University Joseph Ki-Zerbo, Ouagadougou, Burkina Faso.
6Pietro Annigoni Biomolecular Research Center (CERBA), 01 P.O. Box 364, Ouagadougou, Burkina Faso.

*Corresponding Author Email: fantaroued(at)gmail.com

Tel.+226 70 55 70 19

Razacswendé Fanta OUEDRAOGO

Cyrille BISSEYE

Kouka Fidèle TIENDREBEOGO

Hemayoro SAMA

Makido OUEDRAOGO

Gérard ZOMBRE

Jacques SIMPORE


Article Number: IJAPR.21.019  |   Pages: 165-172  |   Vol. 9 (6), November 2021   |   DOI: https://doi.org/10.15739/IJAPR.21.019

 Received: September 28, 2021  Accepted: November 5, 2021  Published: November 8, 2021

Abstract

This study aims to evaluate the expression of genes implicated in three Jatropha curcas ecotypes mechanisms of protection against drought in Burkina Faso. Three drought component factors (sunlight intensity, water deficit, and sunlight associated with water deficit) and three genes including APX1 (cytosolic ascorbate peroxidase), JcHSP1 and JcHSP2 (heat shock proteins) were considered. Plants were grown until 33 days after sowing in a mesh shelter with the translucent plastic roof (at 122 µmol photons.m-2.s-1) and receiving 1 liter of tap water every three days underwent three separate treatments for 20 days: direct sunlight at 572 µmol photons.m-2.s-1, watering suspension, and a treatment was combined the two previous ones. Gene expression at the leaf level was sought by reverse transcription and visualized by electrophoresis on the agarose gel. The results showed a conditioned overexpression of genes. APX1 was associated with water stress and ecotype. JcHSP1 and JcHSP2 were related to all environmental factors, while JcHSP2 was also associated with ecotype. We have also shown a predominance of the water stress effect on gene expression. Our results suggest that APX1, JcHSP1 and JcHSP2 genes are involved in the adaptive molecular response of J. curcas ecotypes to drought and could be “positive” molecular markers for water stress.

Keywords:

Jatropha curcas, Ecotype, Sunlight, Water deficit, RT-PCR.

How to Cite this Article

Ouedraogo RF, Bisseye C, Tiendrebeogo KF, Sama H, Ouedraogo M, Zombre G, Simpore J(2021). Expression of APX1, JcHSP1 and JcHSP2 genes in three Jatropha curcas L. ecotypes under light and water stress in Burkina Faso. Int. J. Agric. Pol Res. 9(6):165-172.

Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.


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