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Original Research Article

The effect of sub-anesthetic doses of ketamine on plasma oxidative stress in pilocarpine-induced epilepsy in mice

Fatma Tannich1,2, Sonia Hamlaoui2 and Ouajdi Souilem*1


1Laboratory of Physiology and Pharmacology, National School of Veterinary Medicine, Sidi Thabet, University of Manouba, Tunisia.
2Neurophysiology Laboratory and Functional Pathology, Department of Biological Sciences, Sciences Faculty of Tunis, University Campus El-Manar, Tunis, Tunisia.

*Corresponding Author Email: labanimal2004(at)yahoo.fr

Tel: +21652444235;+21697087745


Article Number:  |   Pages:  |     |   DOI:

 Received: October 16, 2019  Accepted: December 4, 2019  Published: December 10, 2019

Abstract

The aim of this study was to determine the efficacy of sub-anesthetic doses of ketamine on plasma oxidative stress in pilocarpine-induced epilepsy in mice. Mice received either 0.9% saline (control group), or pilocarpine (100 mg/kg every 20 minutes), or ketamine (10 mg/kg every 30 minutes), or ketamine that was administrated after status epilepticus (SE) at the same doses. Blood samples were collected for the determination of oxidative stress parameters and antioxidant enzymes. Data showed that pilocarpine-induced drastic oxidative stress characterized by high lipid oxidation (+202%, p<0.01), the increase of nitric oxide (+255%, p=0.01) and decrease in antioxidant enzyme activities catalase (-36%, p<0.01), peroxidase (-170%, p<0.01) and superoxide dismutase (-35%, p=0.01). Significant reduction of the non-enzymatic antioxidant uric acid (+232%, p=0.03) was realized. Pilocarpine also increased glucose (+213%, p<0.01). Ketamine had the reverse effect and counteracted almost all pilocarpine-induced deleterious impacts around control levels. In conclusion, post-treatment with ketamine exerts a decrease in oxidative damage associated with a decrease in lipid peroxidation and an increase in antioxidant defenses of plasma in mice model of epilepsy.

Keywords:

Epilepsy, ketamine, oxidative stress, pilocarpine, plasma, mice

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