Issues in Biological Sciences and Pharmaceutical Research

Original Research Article

Response of pepper (Capsicum annuum) cultivars from Benin for somatic embryogenesis from callus

Montcho Hambada K. David1*, Kokouvi Tony1, Hodehou D. A. Thierry1, Baba Crescent1, Houndjo Jean Luc2 and Gandonou Christophe Bernard2

1Ecole de Gestion et de Production Végétale et Semencière, Université Nationale d’Agriculture, BP : 43 Kétou, Bénin
2Laboratoire de Physiologie Végétale et d’Etude des stress environnementaux, Université d’Abomey-Calavi, Bénin

*Corresponding Author Email: montchodav(at)

Tel: (+229) 95767060

Montcho Hambada K. David

Kokouvi Tony

Hodehou D. A. Thierry

Baba Crescent

Houndjo Jean Luc

Gandonou Christophe Bernard

Article Number: ibspr.21.006  |   Pages: 48-53  |   Vol. 9 (2), March 2021   |   DOI:

 Received: January 12, 2021  Accepted: March 3, 2021  Published: March 14, 2021


The screening of resistant cultivars of pepper against environmental stress, such as drought and salinity, is a challenge to be addressed in Benin. However, it needs to be done in order to improve production and with regard to the importance of pepper in the country. This study was designed to evaluate the response of three selected pepper cultivars (Adologbo, Gbatakin, and TPS0251) to the production of calluses with a view to a large multiplication by somatic embryogenesis. The production of a large number of uniform materials is also essential for the selection of salinity-resistant varieties. Young leave explants collected from plants aged 15, 21 and 30 days after transplantation were grown on Murashige and Skoog mineral-based mediums complemented by 2,4-D and BAP at 2 mg/L and 1,86 mg/L respectively. Four weeks after cultivation, it was observed that the rate of induction of callus was not genotype-dependent and was not higher than 50 per cent on average for any cultivar of pepper. Callus was obtained only from explants collected from 30-day-old plants and all of the three cultivars were embryonic. These results will help as a basis to boost the production of plant material through somatic embryogenesis, which will help in the selection of resistant against cultivars.


Callus induction, micro-propagation, embryogenesis, salinity

How to Cite this Article

Montcho HKD, Kokouvi T, Hodehou DAT, Baba C, Houndjo JL, Gandonou CB(2021). Response of pepper (Capsicum annuum) cultivars from Benin for somatic embryogenesis from callus. Issues Biol. Sci. Pharma. Res. 9(2):48-53.

Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.

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