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Ataikiru, T. L
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Original Research Article

Hydrocarbon biodegradation and antibiotic sensitivity of microorganisms isolated from an oil polluted site in Kokori, Delta State, Nigeria

Ataikiru, T. L1*, Okorhi-Damisa, F. B1 and Onyegbunwa, A. E1


1Department of Environmental Management and Toxicology, Federal University of Petroleum Resources, Effurun, Delta State, Nigeria.

Ataikiru, T. L

Okorhi-Damisa, F

Onyegbunwa, A. E


Article Number: ibspr.20.005  |   Pages: 43-50  |   Vol.8 (2), April 2020   |   DOI: https://doi.org/10.15739/ibspr.20.005

 Received: March 20, 2020  Accepted: April 15, 2020  Published: May 20, 2020

Abstract

This study aimed at assessing hydrocarbon biodegradation and antibiotic resistance among bacterial isolates from petroleum polluted and pristine soil samples. Standard microbiological methods were used to isolate, characterize and identify bacterial isolates from the soil samples. Hydrocarbon degradation potential of the isolates were determined using crude oil (1%) as the sole hydrocarbon source and redox reagent (2, 6 – dichlorophenol indophenol, 2%). Also, the standard disc diffusion method was used to test for antibiotic susceptibility pattern of the isolates. Ten bacterial isolates were obtained from soils and identified as species of Lactobacillus, Micrococcus, Enterococcus, Enterobacter, Azomonas, Proteus, Pseudomonas, Acinetobacter, Bacillus and Escherichia coli. All bacterial isolates except Escherichia coli had crude oil bio-degradative capabilities. Isolates with zone of inhibition less than or equal to 12mm (≤ 12mm) were regarded as resistant while those greater than 12mm (> 12mm) were susceptible to the respective antibiotics. Bacillus had multiple resistance to antibiotics (norfloxacin, amoxil, streptomycin and ampiclox) while Micrococcus and Lactobacillus were most susceptible amongst the Gram positive bacteria. Also, Pseudomonas showed multiple resistance to ampicillin, ceporex and gentamycin while Escherichia coli was the most susceptible of the Gram negative bacteria. On the other hand, in relating the hydrocarbon degradation capabilities and antibiotic resistance pattern, among the bacterial isolates from the crude oil polluted soil and pristine soil samples, Lactobacillus, Micrococcus, Enterobacter, Azomonas and Acinetobacter were able to degrade crude oil and did not show multiple resistance to the antibiotics tested. Hence, they could be valuable in the bio-treatment of crude oil polluted environs, as their use does not result to any public health or environmental risk relating to the spread of resistance genes.

Keywords:

Antibiotic sensitivity discs, hydrocarbons, Gram positive, Gram negative bacteria, resistant, susceptibility

How to Cite this Article

Ataikiru TL, Okorhi-Damisa FB, Onyegbunwa AE (2020). Hydrocarbon biodegradation and antibiotic sensitivity of microorganisms isolated from an oil polluted site in Kokori, Delta State, Nigeria. Issues Biol. Sci. Pharma. Res.8(2):43-50.

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