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Original Research Article

Histological study on the effect of aqueous extract of citron leaf on pancreas of hyperglycemic wistar rats

Uthman Ademola Yusuf1*, Gibson Sijumbila1, Samuel Bolaji Mesole2, John Amos Mulemena1, Kingsley Kamvuma1,Olugbenga Olawale Eweoya3 and Thomas Kehinde Adenowo4

1Department of Anatomy, School of Medicine and Health Sciences, Mulungushi University, Livingstone Campus, Zambia.
2Department of Anatomy, Texila American University, Lusaka, Zambia.
3Department of Anatomical Sciences, University of Abuja. Nigeria.
4Anatomy Department, Faculty of Basic Medical Sciences, College of Health Sciences, Olabisi Onabanjo University, Sagamu Campus, Nigeria.

*Corresponding Author Email: uthmanademola(at)

Article Number:  |   Pages:  |     |   DOI:

 Received: September 26, 2019  Accepted: November 22, 2019  Published: December 10, 2019


The present study was carried out to assess some effects of Citrus medica leaf extract treatment on the following parameters in hyperglycemic Wistar rats: diabetic hyperglycaemia and microanatomy of pancreas. The male adult Wistar rats (40) weighing between 160-200g were indiscriminately classified into four consisting of 10 rat namely: (A) normal control, (B) hyperglycemic only, (C) hyperglycemic treated with citron and (D) citron only. Aqueous leaf extracts of citron were administered orally at 400mg/kg body weight daily for 6 weeks. After 6weeks extract of administration, animals were sacrificed by cervical dislocation. Organ weight was also taken while the pancreas was processed for light microscopy. Data were analysed using one way ANOVA. p<0.05 was considered significant. After 6 weeks the glucose level of hyperglycemic+Citron and Citron only groups significantly lowered, increase in body weight and relative pancreatic weight were observed relative to the hyperglycemic group. The histological examination of the pancreas of hyperglycaemic rats showed reduction Islet of Langerhans with fibrotic tissue while hyperglycemic+Citron rats was similar to control rats. Aqueous leaf extract of citronhas the ability to ameliorate the damage initiated by hyperglycemia on the pancreas of adult male Wistar rats.


Pancreas, streptozotocin, Citrus medica, hyperglycemia, Wistar rat.

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