Evaluation of two phytoextracts for induction of phenolic markers indicative of the physiological status improvement of cotton grown in Côte d’Ivoire

Abstract

The efficiency of exogenous application of sugarcane stem filtrate (FCS) and cotton stem filtrate (FTC) was investigated on cotton to stimulate phenol biosynthesis and to identify phenol markers of good physiological status of plant. The stem residues were dried and 500g were ground. The resulting powder was dissolved in 1 L of water and represents the hytoextract solution at 100%. The sugarcane stem filtrate (FCS) was prepared at 25% and the cotton stem filtrate (FTC) at 20% in this study. After spraying the cotton leaves, quantitative and qualitative analysis of phenols were evaluated as well as the physiological status of the plants through the determination of total chlorophyll. The results showed the ability of phytoextracts to promote phenol accumulation in cotton. FTC was the most effective treatment because the phenol content was higher than FCS. Furthermore, UHPLC analysis revealed that caffeyl-5 quinic acid and rutin as well as isoquercetin and astringin, induced only after application of FCS and FTC respectively, are phenol markers of good physiological status in cotton. FCS and FTC had beneficial effects on cotton-leaf chlorophyll content suggesting a biocontrol role. In fact, the application of FTC favored an intense activation of metabolic functions than FCS. The observed of good plant health status was related to phenol, which are responsible for developing disease resistance skills. Thus, the stimulation of natural defenses could be used as an alternative to chemical control of pathogens improving productivity while respecting the environment and the health of farmers. FTC can be recommended for the stimulation of defenses in cotton.

Keywords:

Chlorophyll, cotton, FCS, FTC, phenol, physiological status, polysaccharide