Issues in Biological Sciences and Pharmaceutical Research

Original Research Article

Cytotoxic activities of Psidium guajava and Mangifera indica plant extracts on human healthy skin fibroblasts and human hepatocellular carcinoma

Gislaine Aurelie Kemegne1,2*, Nadir Bettache3, Maximilienne Ascension Nyegue1, François-Xavier Etoa1 and Chantal Menut3

1Department of Microbiology, University of Yaoundé I, Yaoundé, Cameroon PO Box 812
2Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, Yaoundé, Cameroon PO Box 13033
3Université de Montpellier, IBMM-UMR 5247 CNRS-UM, Equipe glyco et nanovecteurs pour le ciblage thérapeutique, 15 avenue Charles Flahault, BP 14491, FR-34093 Montpellier Cedex 5, France

*Corresponding Author Email: kaurelig(at)

Tel. +237 69900296

Gislaine Aurelie Kemegne

Nadir Bettache

Maximilienne Ascension Nyegue

François-Xavier Etoa

Chantal Menut

Article Number: ibspr.20.007  |   Pages: 58-64  |   Vol. 8 (4), September 2020   |   DOI:

 Received: May 12, 2020  Accepted: July 15, 2020  Published: September 11, 2020


This study aimed to evaluate the cytotoxic activity of aqueous, ethanol extracts and essential oils (EO) obtained from Psidium guajava leaf and Mangifera indica bark by the MTT assay. The cytotoxic effect of the samples against healthy skin human fibroblasts (CCD45-SK) and hepatocellular carcinoma cells (HepG2) was evaluated using the colorimetric MTT reduction assay, which analyses the number of viable cells, cultured in 96-cells microplates, by the cleavage of tetrazolium salt added to the culture medium (samples concentrations ranging from 0.005 to 0.2 mg/mL). An inhibition of CCD-45 SK cells proliferation generated by inhibitory concentrations of 0.1 and 0.063 mg/mL was observed with the EO obtained from P. guajava and M. indica respectively against 0.1 and 0.08 for HepG2 cells. HepG2 cells proliferation was decreased by inhibitory concentrations of P. guajava leaves extracts between 0.006 and 0.013 mg/mL while concentrations were between 0.065 to 0.1 mg/mL for M. indica bark extracts. Interesting, the two EO were cytotoxic on CCD-45 SK and HepG2 cells while aqueous and ethanol extracts were cytotoxic only on HepG2 cells. Finally, our results suggest that the aqueous and ethanol extracts from P. guajava and M. indica could be used as cytotoxic agent in human medicine.


Psidium guajava, Mangifera indica, plant extracts, CCD-45 SK, HepG2, MTT assay, cytotoxic properties

How to Cite this Article

Kemegne GA, Bettache N, Nyegue MA, Etoa FX, Menut C (2020). Cytotoxic activities of Psidium guajava and Mangifera indica plant extracts on human healthy skin fibroblasts and human hepatocellular carcinoma.Issues Biol. Sci. Pharma. Res.8(4):58-64.

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