Issues in Biological Sciences and Pharmaceutical Research

Original Research Article

Comparison of 2 HIV1 RNA VL measurement commercial kits in Cote d’Ivoire

Kone-Dakouri Yekayo1*, Kone-Kone Fatoumata1,2, Aka Tano Cyrielle2, Yapo Vincent De Paul2, Toni Thomas D. Aquin2, Ahiboh Hugues1,2, Blidieu Juste2, Yayo Sagou Eric1, Edjeme Ake Angele1, Attoungbre-Hauhouot Marie Laure1 , Menan Hervé2 and Monnet Dagui1

1Department of Biochemistry, Molecular Biology, Reproduction Biology and Medical Pathologies, Unit of Education and Research of Pharmaceutical and Biological Sciences, Felix Houphouet Boigny University, Abidjan, Ivory Coast
2Diagnostic and Research Center on AIDS and Other Infectious Diseases (CeDReS), Treichville, University Hospital, Abidjan, Ivory Coast.

*Corresponding Author Email: bene.yekayo(at)

Tel.:+225 0707220143

Kone-Dakouri Yekayo

Kone-Kone Fatoumata

Aka Tano Cyrielle

Yapo Vincent De Paul

Toni Thomas D. Aquin

Ahiboh Hugues

Blidieu Juste

Yayo Sagou Eric

Edjeme Ake Angele

Attoungbre-Hauhouot Marie Laure

Menan Hervé

Monnet Dagui

Article Number: ibspr.21.009  |   Pages: 86-92  |   Vol. 8 (3), October 2021   |   DOI:

 Received: July 26, 2021  Accepted: September 15, 2021  Published: October 10, 2021


The goal of this study was to compare the two operational platforms (Biocentric and Roche CAP/CTM) in the country to assess their degrees of concordance in order to avoid interruptions in the virological follow-up of people living with HIV (PLHIV) in Côte d’Ivoire. HIV1 RNA viral load (VL) of 182 plasmas were measured by means of the 2 platforms in the CeDReS laboratory. The comparison was made by concordance, Bland-Altman (BA) and the appreciation of clinical differences between the measured VL. The proportions of suppressed viral loads (76% and 75%) were similar on the 2 platforms. The mean VLs were 3.43 and 3.66 Log10 /mL respectively on CAP/CTM and Biocentric. According to BA, the bias was 0.23 Log10 in favour of Biocentric and the correlation coefficient of agreement (0.922) was poor. The Kappa coefficients of 0.846 at the detection thresholds and 0.878 at the viral suppression threshold indicated a near perfect level of agreement. Clinically significant differences (> |0.6|) were observed between clinically equivalent VL, with no influence on management. Biocentric and CAP/CTM had good agreement but the power of agreement was low. Clinical differences were not significant, however by interchanging these platforms, communication between biologists and clinicians is useful.


HIV1 RNA VL, biocentric, Roche CAP/CTM, concordance, clinical difference

How to Cite this Article

Yekayo KD, Fatoumata KK, Cyrielle AT, Paul YV De, Aquin TTD, Ahiboh H, Blidieu J, Yayo SE, Edjeme AA, Attoungbre-Hauhouot ML, Menan H, Monnet D (2021). Comparison of 2 HIV1 RNA VL measurement commercial kits in Cote d’Ivoire. Issues Biol. Sci. Pharma. Res. 9(3):86-92.

Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.

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