Issues in Biological Sciences and Pharmaceutical Research

Original Research Article

Bioactivity guided fractionation of the seeds of Allanblackia floribunda Oliv. (Guttiferae)

Grace A. Akpanika1, Ana Winters2, Gloria A. Ayoola3, Effiong E. Nkanor4 and Barbara Hauck2


1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Calabar, Nigeria
2Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Gogerddan, Aberystwyth SY23 3EB, UK.
3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Lagos, Nigeria.
4Department of Pharmacology, Faculty of Basic Medical Sciences, University of Calabar, Nigeria.

*Corresponding Author E-mail: gakpanika(at)yahoo.com

Tel.: +2348034075133

Grace A. Akpanika

Ana Winters

Gloria A. Ayoola

Effiong E. Nkanor

Barbara Hauck


Article Number: ibspr.22.005  |   Pages: 24-29  |   Vol. 10 (3), July 2022   |   DOI: https://doi.org/10.15739/ibspr.22.005

 Received: March 23, 2022  Accepted: May 23, 2022  Published: July 6, 2022

Abstract

Six phenolic compounds previously isolated from the seeds of Allanblackia floribunda and identified as (1) Gb-2a glucoside (naringenin-1,3-II,8-eriodictyol glucoside), (2) Morelloflavone glucoside, (3) Gb-2a(naringenin-1,3-II,8-eriodictyol), (4) Volkensiflavone, (5) Morelloflavone and (6) Volkensiflavone glucoside were evaluated for their anti-inflammatory, anti-hyperglycemic and antibacterial activities. Their anti-inflammatory activity was evaluated using Heat induced hemolysis assay and inhibition of albumin denaturation technique, anti-hyperglycemic activity was evaluated using α-amylase and α-glucosidase inhibition assay and antibacterial activities were also evaluated for both gram positive and gam negative bacteria using the micro titer discs. All isolates showed a significant inhibition against the activities of α-amylase and so they have a good potential in the alleviation of post prandial hyperglycemia. Gb-2a glucoside, morelloflavone glucoside and morelloflavone showed better ability to protect the human erythrocyte membrane against lysis compared to Gb-2a, Volkensiflavone and its glucoside. Morelloflavone glucoside showed positive activity against Staphylococcus aureus, Escherichia coli and Mycobacterium smegmatis even though the control drug Kanamycin was more potent (0.137± 0.00, 0.12± 0.00 and 0.13± 0.00 mg/ml). Gb-2a, Morelloflavone and their glucosides showed significant anti-inflammatory, hypoglycemic and antibacterial activities. This explains the use of Allanblackia floribunda traditionally for the alleviation of pains and infection.

Keywords:

Allanblackia floribunda, morelloflavone, volkensiflavone, Gb-2a, anti-inflammatory, antibacterial.

How to Cite this Article

Akpanika GA, Winters A, Ayoola GA, Nkanor EE Hauck B(2022).Bioactivity guided fractionation of the seeds of Allanblackia floribunda Oliv. (Guttiferae).Issues Biol. Sci. Pharma. Res. 10(3):24-29.

Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.


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