Issues in Biological Sciences and Pharmaceutical Research

Original Research Article

Antioxidant activity and phytochemical constituents of Philippine Clitoria ternatea flowers as a potential therapeutic agent against infectious diseases

Rosalinda C. Torres1*, Ma. Rachel V. Parcon1, Harvy Jay N. Esmundo1, Danielle Camille P. Canillo1, and Cyril C. Ramil1

1Standards and Testing Division, Industrial Technology Development Institute (ITDI), Department of Science and Technology, Bicutan, Taguig City, Philippines, 1631

*Corresponding Author Email. rosalindactorres(at)

Rosalinda C. Torres

Ma. Rachel V. Parcon

Harvy Jay N. Esmundo

Danielle Camille P. Canillo

Cyril C. Ramil

Article Number: ibspr.22.003  |   Pages: 12-18  |   Vol. 10 (2), May 2022   |   DOI:

 Received: March 4, 2022  Accepted: April 18, 2022  Published: May 3, 2022


Infectious diseases have always been present throughout human history, in which numerous emerging and re-emerging pathogens have been documented. Various strategies have been established to combat these pathogens: from vaccinations, to optimized drug delivery routes, to natural products such as antioxidants and flavonoids. This study evaluated the antioxidant activity of the ethanolic extract of the anthocyanin-rich Clitoria ternatea flowers, from the Philippines, by analyzing its total phenolic content and DPPH radical scavenging activity. The raw materials used in this study were collected from Victoria, Tarlac, Philippines, which are then macerated and soaked in 95% ethanol for 24 hours before extraction. The extract was then filtered using coarse filter paper and concentrated using a rotary evaporator. The samples were subjected to heavy metals analysis, Total phenolic content and IC50 from DPPH radical scavenging activity of the C. ternatea extract were found to be 3.9519 ± 0.1 mg GAE / 100 g and 53.6913 mg/kg, respectively. Phytochemical screening of the ethanolic extract of C. ternatea revealed the presence of triterpenes, saponins, reducing sugars, tannins, sterols, flavonoids, and alkaloids. Results show the potent antioxidant properties and the abundance of flavonoids of C. ternatea extract which may encourage further studies to evaluate its possible applications as plant-derived antioxidants for the therapeutic management of various infectious diseases.


Antioxidant activity, DPPH radical scavenging activity, phytochemical constituents, Clitoria ternatea

How to Cite this Article

Torres RC, Parcon MRV, Esmundo HJN, Canillo DCP, Ramil CC(2022).Antioxidant activity and phytochemical constituents of Philippine Clitoria ternatea flowers as a potential therapeutic agent against infectious diseases.Issues Biol. Sci. Pharma. Res.10 (2):12-18.

Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.

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