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Original Research Article

Anaerobic co-digestion of food wastes and chicken dung in lab-scale two-stage system

L. A. Slobodkina1*, N.G. Akinshina1, and A. A.Azizov1

1Department of Applied Ecology and Sustainable Development, National University of Uzbekistan Named After Mirzo Ulugbek, University street 4, Tashkent, Uzbekistan, 100174

*Corresponding Author Email:mila-md357(at)

Tel. +998909602816

L. A. Slobodkina

N.G. Akinshina

A. A. Azizov

Article Number: ibspr.21.002  |   Pages: 12-18  |   Vol. 9 (1), January 2021   |   DOI:

 Received: October 12, 2020  Accepted: December 18, 2020  Published: January 4, 2021


The depletion of natural energy resources (oil, gas) and increase in environmental pollution are the most important problems facing humanity. Waste from agricultural enterprises and the municipal sector is harmful to the natural environment. Nowadays one of the most efficient technologies for the processing of livestock manure is anaerobic digestion (AD). One of the main sources of raw materials in Uzbekistan, suitable for extracting biogas, are wastes of livestock-breeding and poultry farming, industrial organic wastes. Therefore, the aim of the work is to study the process of two-stage anaerobic co-fermentation, measure the production and composition of biogas. The laboratory set-up for two-stage co-anaerobic fermentation operated about 55 days. (1-st stage at 55 °C hydrolysis of a substrate containing 60,49gVS; 2-nd stage methane formation at 37 °C; OLRMT(organic loading rate) – 4.8 gCOD/dm3/day; HRT(Hydraulic retention time)-14 days. Our results demonstrated wave-like dynamics. That are associated with the frequency of loading the substrate in hydrolyzer in the first stage and difference in the chemical composition of the first and second pressing hydrolyze. The results showed that most of the organic matter (65%) converted to biogas. The gas composition has been determined and its dynamics studied. The total methane yield in 14 days was 10950.73 ml. 181.03 ml of methane and 80.42 ml of hydrogen released from 1 g of organic waste. It shown that Uzbekistan has a big potential to produce biogas throw co-anaerobic digestion.


Anaerobic digestion, co-fermentation, two stage AD, organic waste, biogas yield, biogas composition, chicken dung

How to Cite this Article

Slobodkina LA, Akinshina NG, Azizov AA(2021). Anaerobic co-digestion of food wastes and chicken dung in lab-scale two-stage system. Issues Biol. Sci. Pharma. Res. 9(1):12-18.

Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.

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