Issues in Biological Sciences and Pharmaceutical Research

Original Research Article

Acute and subchronic toxicity assessment of the dichloromethane / methanol stem bark extract from Erythrina senegalensis DC (Fabaceae) in Wistar rats

Fofana Souleymane1*, Kouama K. Benjamin2, Da Ollo3, Yerbanga R. Serge2, Ilboudo Sylvain4, Ouédraogo Noufou4, Ouédraogo Moussa5 and Guissou I. Pierre6


1Laboratoryof Drug Science. Higher Institute of Health Sciences (INSSA) / Nazi BONI University. Burkina Faso.
2Laboratory of Pharmacology. Health Sciences Research Institute (IRSS). Burkina Faso.
3Laboratory of Biophysics and biochemistry. Higher Institute of Health Sciences (INSSA) / Nazi BONI University. Burkina Faso.
4Institute of Research in Health Sciences (IRSS / CNRST)
5Laboratory of Drug Development (LADME) / Training and Research Unit / Health Sciences, Joseph KI-ZERBO University
6Faculty of Health Sciences. Saint Thomas d’Aquin University.

*Corresponding Author E-mail: fof_soul(at)yahoo.fr

Tel. +226 61 01 05 40.

Fofana Souleymane

Kouama K. Benjamin

Da Ollo

Yerbanga R. Serge

Ilboudo Sylvain

Ouédraogo Noufou

Ouédraogo Moussa

Guissou I. Pierre


Article Number: ibspr.21.003  |   Pages: 19-26  |   Vol. 9 (1), January 2021   |   DOI: https://doi.org/10.15739/ibspr.21.003

 Received: October 5, 2020  Accepted: December 15, 2020  Published: January 4, 2021

Abstract

Erythrina senegalensis is a medicinal plant used in sub-Saharan Africa to treat pain, asthenia, inflammation, jaundice, malaria and infections. This work aimed to study the phytochemistry, the acute and subchronic toxicity of the dichloromethane / methanol stem bark extract from E. senegalensis. The phytochemical groups have been characterized in the extract. The content of total polyphenols and flavonoids was determined using the colorimetric methods of Folin-Ciocalteu and aluminum trichloride respectively. Acute toxicity was assessed in NMRI mice and the 28-day subchronic toxicity in Wistar rats by oral gavage. Total polyphenols and flavonoids had a high content in the extract. The DCM / MeOH extract, up to 1200mg / Kg of body weight (bw), did not cause any mortality or modification of the behavior of the treated mice. In the subchronic toxicity study by oral administration of 60 and 120mg / Kg of bw of extract, neither the relative weight nor the biological parameters of renal or hepatic impairment were significantly different from a group treated with the control group (p > 0.05). Anatomopathological examination revealed no signs of toxicity of the extract on the heart, lungs, spleen, liver and kidney. E. senegalensis is rich in polyphenols. The doses administered did not induce any significant toxicity.

Keywords:

E. senegalensis; Total polyphenolic content; Acute toxicity ; Subchronic toxicity

How to Cite this Article

Fofana S, Kouama KB, Da O, Yerbanga RS, lboudo S, Ouédraogo N, Ouédraogo M, Guissou IP(2021). Acute and subchronic toxicity assessment of the dichloromethane / methanol stem bark extract from Erythrina senegalensis DC (Fabaceae) in Wistar rats. Issues  Biol. Sci. Pharma. Res. 9(1):19-26.

Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.


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