International Journal of Agricultural Policy and Research
Vol.2 (3), pp.098-104, March 2014
Article ID JPR022/14/07 pages
Copyright © 2014 Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 3.0 International License
Original Research Paper
Composition and diversity of endophytic bacterial communities in noni (Morinda citrifolia L.) seeds
Accepted 14 February, 2014
*1Liu Yang, 1Cheng Chi 1Yao Su, 2Xu Pengpeng , 1Cao Yanhua, 1Li Jinxia , 1Wang Jiemiao,3Tan Wangqiao
1China National Research Institute of Food and Fermentation Industries, Beijing 100015, People’s Republic of China
2Beijing Geneway Technology Co., Ltd., Beijing 100010, People’s Republic of China.
3Hainan Noni Biological Engineering Development Co., Ltd., Haikou 570125, People’s Republic of China.
*Corresponding Author Email: ly81150(at)163.com
The objective of this study is to investigate the endophytic bacterial communities in Noni (Morinda citrifolia L.) seeds. By using high-throughput sequencing (HTS) technique, a research on the diversity of endophytic bacterial communities in Noni seed samples was conducted by this paper, which were collected from four planting locations in the introduction and cultivation base of Hainan Noni Biological Engineering Development Co., Ltd. in Sanya. A total of 268159 sequences and 2870 Operational Taxonomic Units (OTUs) were obtained from the seed samples, which were classified into N1, N2, N3 and N4, through HTS by bioinformatics analysis. The results showed that there was a good consistency between bacterial community structure and distribution of dominant genus in all samples. A mount of 10 OTUs of the most abundance associated with the four sample libraries were found to be related to Pelomonas sp., Ochrobactrum intermedium, Kinneretia sp., Serratia sp., Paucibacter sp., Leptothrix sp., Inhella sp., Ewingella sp., Thiomonas sp. and Massilia sp.. This study has laid a solid foundation for further study in beneficial microbes, which would help to improve the growth and efficacy of component production in Noni plants.
Key words: Noni Seed, endophytic bacteria, bacterial community, HTS