International Journal of Agricultural Policy and Research
Vol.3 (9), pp. 341-350, September 2015
Available online at https://www.journalissues.org/IJAPR/
Article 15/ID/JPR070/10/ pages
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.
Original Research Article
Isolation and potential of Ochrobactrum sp. NW-3 to increase the growth of cucumber
Song Xu1, Ying Liu2, Jingjing Wang1, Tingting Yin1,3, Yifan Han1, Xingbiao Wang1 and Zhiyong Huang1*
1Applied Microbial Ecology Engineering Lab, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
2College of Natural Resources and Environment, South China Agricultural University,Guangzhou, Guangdong, 510642, China.
3College of Biology Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China.
*Corresponding Author E-mail: huang_zy(at)tib.cas.cn
‘Biofertilizers’ have been defined as alternative substances, to chemical fertilizers, which can stimulate plant growth and increase yield. Plant growth promoting bacteria (PGPB) represent the main component of biofertilizers. We chose seven bacteria with higher nitrogenase activity, from twenty-nine nitrogen fixing bacteria isolated from different natural environments, to evaluate the effects of bacteria inoculation on cucumber under controlled (pot) conditions. Furthermore, growth promoting characteristics (indole-3-acetic acid (IAA), ACC-deaminase activity, siderophores production, phosphorus solubilization and potassium solubilization ability) were evaluated for seven strains and identification of strains was carried out by amplification, sequencing and comparison in GenBank database for 16S rDNA genes. Ochrobactrum sp. NW-3 had a high ability to increase the growth of cucumber in pot experiments and this strain showed the five factors evaluated to stimulate the plant growth. For this reason, Ochrobactrum sp. NW-3 was used to perform a field experiment in Anshan, Liaoning Province, the main local economic for cucumber production. Cucumber yield and quality increased significantly after three months of field experiment, suggesting that Ochrobactrum sp. NW-3 can exert promoting growth effect under the complex field environment. It is the first report on the use of Ochrobactrum sp. for stimulating cucumber growth and yields successfully in field experiments.
Key words: Biofertilizer, Ochrobactrum sp. NW-3; plant growth promoting bacteria, diverse mechanisms, cucumber field application.