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E Ughala
CJ Ogugbue
GC Okpokwasili

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E Ughala
CJ Ogugbue
GC Okpokwasili

International Research Journal of Public and Environmental Health
Vol.6 (8),pp. 210-218, December 2019
ISSN 2360-8803
Available online at
Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.

Original Research Article

Enhanced remediation of a long term crude oil polluted soil using soya bean and poultry residues

E. Ughala*1, C. J. Ogugbue1, and G. C. Okpokwasili1

1Department of Microbiology, University of Port Harcourt, PMB 5323, Port harcourt, Nigeria.

*Corresponding Author Email: eawujo(at)

Tel. +2347034864001

date Received: November 8, 2019     date Accepted: December 6, 2019     date Published: December 9, 2019


The aim of this study is to evaluate the effect of soy bean and poultry residues and its applicability in the remediation of a long term crude oil contaminated soil. Crude oil contaminated and uncontaminated soil samples were randomly collected at 0-30 cm depth from subsurface horizon soil. The controlled experimental setups with different nutrient formulations were exposed to natural weather conditions for 365 days. Crude oil-contaminated soil samples were collected from Bodo West community in Gokana Local Government Area of Rivers State at sites. The Crude oil impacted soil sample was put into six vessels, four of which contained different nutrient amendments (25g of each nutrient formulation) and two untreated controls. Soybean, poultry droppings, NPK fertilizer and a combined nutrient formulation served as the sources of nutrients. The inorganic NPK fertilizer was observed to be more effective in the proliferation of total heterotrophic bacterial population. However, the application of these nutrient sources in combined form was found to be more effective than their individual application. All the sources of nutrient were observed to significantly (p< 0.05) induce bacterial proliferation and enhanced the natural attenuation of hydrocarbon pollutants in the soil, compared to the control sample. The efficacy of soy bean and poultry residues as organic nutrients as well as their application in enhancing natural attenuation has been determined from this study. The combined nutrient formulation was observed to be more efficient than the single application of the nutrient sources. These findings are crucial in developing a large scale strategy for field-based applicationsin this long termcrude oil polluted region. This research therefore provides crucial insight for future projects involving optimization of nutrient utilization and degrading abilities of indigenous microbial population.

Key words: Polluted soil, enhanced remediation, soy bean, poultry residue, crude oil.

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