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Issues in Biological Sciences and Pharmaceutical Research
Vol.6(2),pp.17-22, September 2018
ISSN 2350-1588
Available online at
Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.

Original Research Article

Antimicrobial activity and quantiitative analysis of Ocimum Gratissimum on some pathogenic bacteria

*1Ubafie Martha Oke and 1Ejale Angela. U

1Department of Plant Biology and Biotechnology, University of Benin, Benin-City, Nigeria.

*Corresponding Author Email: ubafie9(at)

date Received: June 4, 2018     date Accepted: July 23, 2018     date Published: September 21, 2018


Ocimum gratissimum commonly called scent leaf is a herbaceous plant belong to the family of lamiacea . It is a well-known medicinal plant amongst farmer and rural dwellers in Nigeria. An investigation of the antimicrobial activity and phyto constituent of Ocimum gratissimum root and leaf was conducted against some clinical isolates of Pseudomonas auroginosa, Klebsiella sp , Bacillus subtilis and type culture of Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC20923). The water extract of both root and leaf were effective against the test organisms. They had no effect on Bacillus subtilis and Pseudomonas auroginosa. The ethanol extract of the root showed no action against the test organisms because no zone of inhibition was observed. The effect of aqueous (water) and ethanol extracts of the leaves and roots of Ocimum gratissimum were compared with a standard antibiotic (ciprofloxacin). The result showed that the extracts had a strong antimicrobial activity. The minimum inhibitory concentrations (MIC) ranges from 5mg/ml to 20mg/ml. Ocimum gratissimum, possesses some important phytochemicals which includes alkaloids, tannins, saponins, flavonoids, and quercetin.

Key words: Malaria, Plasmodium falciparum, Plasmodium vivax, apical membrane antigen, vaccine candidates, AMA1 alleles, polymorphism

Ubafie and Ejale