Issues in Biological Sciences and Pharmaceutical Research
Vol.7(1), pp. 1-6, January 2019
Available online at https://www.journalissues.org/IBSPR/
Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.
Original Research Article
Hepatoprotective effect of a Tagtaggunsel on carbon- tetrachloride-induced liver fibrosis
1,2*Tsogtoo B, 3Dejidmaa B, 3Chimedragchaa CH, 4Nyamdolgor U, 5Badamsuren D and 1Tserendagva D
1Department of Theory of Mongolian medicine, School of International Mongolian medicine, Mongolian National University of Medical Sciences, 14210 Ulaanbaatar, Mongolia.
2Nei Mongolian National University, China.
3Research Center, Institute of Traditional Medicine and Technology, 17032 Ulaanbaatar, Mongolia.
4Pathology Department, Institute of Veterinary Medicine, Mongolian State University of Life Sciences University, 17024 Ulaanbaatar, Mongolia.
5Department of gastroenterology, Shastin Central Hospital 16064, Ulaanbaatar, Mongolia.
*Corresponding Author Email: b.tsogtoo2018(at)yahoo.com
In Mongolia and worldwide, hepatic disease remains one of the leading causes of population mortality. The aim of the present study was to investigate the effect of a Mongolian medicine formula, Tagtaggunsel (TTG), on the treatment of liver fibrosis in rats. Rats were divided into 4 groups of 15 animals each. 1. Healthy group, 2. Control groups rats were intoxicated with CCl4 i.p injection (10%, 0.1 ml/kg body weight CCl4/olive oil, three weekly for 12 weeks), 3. Treatment group rats received CCl4 i.p. and TTG orally (100 mg/kg daily) and 4. Comparative group rats received CCl4 i.p. and Silymarin orally (100 mg/kg, daily). The hepatoprotective effect was assessed estimation of serum concentration of ALT, AST, albumin and 2 types of antioxidant markers-Lipid peroxidase, Superoxidismutase (LPO, SOD) Histopathological changes in the liver were assessed using hematoxylin and eosin staining, Masson-trichrome. Showed that TTG significantly serum level AST, ALT and ALP but Albumin increased compared to CCL4 intoxicated group. In addition TTG significantly reduced the level of LPO, but SOD significantly increased compared to CCl4 intoxicated group. These findings were confirmed with the histopathological observations, TTG had effect of anti inflammatory processes and decreased the connective tissue CCl4 on liver cells compared to that observed in CCl4-intoxicated rats. In conclusion TTG had potential hepatoprotective effects at 100 mg/kg.
Key words: Tagtaggunsel, carbon tetrachloride, hepatoprotective, silymarin, liver fibrosis, LPO, SOD