Issues in Biological Sciences and Pharmaceutical Research
Vol.7(5),pp.106-116, December 2019
Available online at https://www.journalissues.org/IBSPR/
Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.
Original Research Article
The effect of sub-anesthetic doses of ketamine on plasma oxidative stress in pilocarpine-induced epilepsy in mice
Fatma Tannich1,2, Sonia Hamlaoui2 and Ouajdi Souilem*1
1Laboratory of Physiology and Pharmacology, National School of Veterinary Medicine, Sidi Thabet, University of Manouba, Tunisia.
2Neurophysiology Laboratory and Functional Pathology, Department of Biological Sciences, Sciences Faculty of Tunis, University Campus El-Manar, Tunis, Tunisia.
*Corresponding Author Email: labanimal2004(at)yahoo.fr
The aim of this study was to determine the efficacy of sub-anesthetic doses of ketamine on plasma oxidative stress in pilocarpine-induced epilepsy in mice. Mice received either 0.9% saline (control group), or pilocarpine (100 mg/kg every 20 minutes), or ketamine (10 mg/kg every 30 minutes), or ketamine that was administrated after status epilepticus (SE) at the same doses. Blood samples were collected for the determination of oxidative stress parameters and antioxidant enzymes. Data showed that pilocarpine-induced drastic oxidative stress characterized by high lipid oxidation (+202%, p<0.01), the increase of nitric oxide (+255%, p=0.01) and decrease in antioxidant enzyme activities catalase (-36%, p<0.01), peroxidase (-170%, p<0.01) and superoxide dismutase (-35%, p=0.01). Significant reduction of the non-enzymatic antioxidant uric acid (+232%, p=0.03) was realized. Pilocarpine also increased glucose (+213%, p<0.01). Ketamine had the reverse effect and counteracted almost all pilocarpine-induced deleterious impacts around control levels. In conclusion, post-treatment with ketamine exerts a decrease in oxidative damage associated with a decrease in lipid peroxidation and an increase in antioxidant defenses of plasma in mice model of epilepsy.
Key words: Epilepsy, ketamine, oxidative stress, pilocarpine, plasma, mice