Issues in Biological Sciences and Pharmaceutical Research
Vol.7(3),pp.58-63, September 2019
Available online at https://www.journalissues.org/IBSPR/
Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.
Original Research Article
Chemical composition and evaluation of anticholinesterase activity of essential oil from Cameroonian propolis
Alfred Ngenge Tamfu*1,2,4, Maurice Tagatsing Fotsing1, Emmanuel Talla3, Mehmet Ozturk2,Joseph Tanyi Mbafor1, Mehmet Emin Duru2 and Farzana Shaheen4
1Department of Organic Chemistry, University of Yaounde 1, Cameroon
2Department of Chemistry, Mugla Sitki Kocman University, Turkey
3Department of Materials Engineering, School of Chemical Engineering and Mineral
Industries, University of Ngaoundéré, Cameroon.
4H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi – 75270 Pakistan
*Corresponding Author Email: macntamfu(at)yahoo.co.uk
Limited studies exist on tropical propolis volatile constituents. Hydrodistillation of a Cameroonian propolis sample afforded a light-yellow oil with a yield of 0.04% (v/w). Chemical characterization using gas chromatography flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS) led to the identification of 73 compounds representing 98.3% of total oil constituents. The most abundant components were α-calacorene (21.61%), 4,5,9,10-dehydroisolongifolene (5.56%), δ-cadinol (5.36%), 8,8,9-trimethyldeca-3,5-diene-2,7-dione (4.98%), 2-hydroxymethylene-6-isopropyl-3-methylcyclohexanone (4.90%) and γ-himachalene (4.11%). Ellman’s colorimetric assay was used to evaluate anticholinesterase activity of propolis essential oil. Acetylcholinesterase inhibition was moderate with IC50 value of 54.35±1.01 μg/mL compared to 5.01±0.09 μg/mL for galantamine while butyrylcholinesterase inhibition was profoundly high with IC50 value 27.63 ± 0.62 μg/mL compared to the standard drug galantamine with IC50 53.9±0.56 μg/mL. These results show that anticholinesterase activity of propolis volatiles could be a potential solution to Alzheimer’s disease.
Key words: Propolis, essential oil, hydrodistillation, GC-MS, α-calacorene, anticholinesterase activity