International Journal of Agricultural Policy and Research
Vol.2 (12), pp. 468-474, December 2014
ISSN 2350-1561
Article 14/ID/ JPR216/10/ pages
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.

Original Research Article

Addition of lipolytic enzyme in anaerobic co-digestion of swine manure and inclusion levels of waste vegetable oil

Natália da Silva Sunada1, Jorge de Lucas Júnior1, Ana Carolina Amorim Orrico2, Marco Antônio Previdelli Orrico Júnior2, Alice Watte Schwingel2 and Mônica Sarolli Silva de Mendonça Costa3

1Sao Paulo State University – “Júlio de Mesquita Filho” (UNESP) Jaboticabal, SP, Brazil.
2College of Agricultural Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS, Brazil.
3Western Parana State University, Cascavel, PR, Brazil.

*Corresponding Author E-mail: anaorrico(at)
Tel.: +556734102374.

date Received:     date Accepted: November 17, 2014     date Published:


Studies that aim the reusing of agro-industrial wastes have become very important due to the high production of this sector and environmental preservation. The objective of this work was to evaluate the performance of digesters loaded with swine manure, increasing inclusion levels of waste oil and lipolytic enzyme, through the potential of biogas and methane production, as well as reductions in levels of total solids (TS) and volatile solids (VS). Batch digesters were loaded with substrates containing 4% TS, composed of pig manure, waste oil (ratios of 8, 10 and 12% in relation to levels of TS in the substrate) and lipolytic enzyme (0.05, 0.10, 0.15, 0.20 and 0.25% in relation to levels of TS in the substrate), water and inoculum. Maximum reduction of TS and VS were 56.13 and 64.49% respectively and occurred in inclusion levels of 0.15 and 0.13% lipase and 12% oil. The greatest potential for methane for the amounts of TS and VS added (0.23 and 0.29L) were achieved by inclusion of 12% oil and levels of 0.12 and 0.11% lipase, and these values were up to 29.7% higher than yields observed for the lowest inclusion level of enzyme. The inclusion of 12% oil and 0.15% lipase in the composition of substrates containing pig manure improves the yields of methane and reductions in pollutant constituents.

Key words:

Key words: biodigestion, lipase, lipid, methane, swine

da Silva Sunada et al