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MS Selvi
S Sasikumar
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SG Sadasivam

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MS Selvi
S Sasikumar
S Gomathi
P Rajkumar
P Sasikumar
SG Sadasivam

International Journal of Agricultural Policy and Research
Vol.2 (11), pp. 393-405, November 2014
http://dx.doi.org/10.15739/IJAPR.012
ISSN 2350-1561
Article ID/14/JPR196, 013 Pages
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License



Original Research Article

Isolation and characterization of arsenic resistant bacteria from agricultural soil, and their potential for arsenic bioremediation

Mariaraj Sivakumar Selvi, Sundaresan Sasikumar, Sivasamy Gomathi, Prabhakaran Rajkumar, Ponnusamy Sasikumar and Selvam Govindan Sadasivam*

Department of Biochemistry, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021, India.

*Corresponding Author E-mail: drselvamgsbiochem(at)rediffmail.com
Tel.: +91452 2458213



date Received:     date Accepted: October 21, 2014     date Published:


 Abstract

Arsenic (As) is a toxic pollutant released into the environment either by natural phenomena or anthropogenic activities. It is a potent human toxin, which causes many diseases such as diarrhoea, bladder cancer etc. Pesticides are the major source for accumulation of As in the agricultural soils. Currently available bioremediation techniques have major disadvantages such as secondary environmental pollution and are less cost effective. Therefore, the present study was aimed to isolate arsenic resistant bacteria from terrestrial environment Tamil Nadu, South India for their potential applications in bioremediation strategies. From the isolated fifty arsenic-resistant bacteria, two bacterial isolates BC1 and BC2 were taken for further studies due to their higher resistance ability to As. The optimum pH and temperature were found to be at 6 and 37 °C respectively. The 16S rRNA gene sequence of the isolate BC1 belongs to the genera Enterobacter asburiae and BC2 Enterobacter cloacae. The results revealed that our isolates BC1 and BC2 encoded arsenite oxidizing gene aoxA and arsenate reducing gene arsC respectively. Further characterization of aoxA gene and arsC gene will be useful for the development of efficient bioremediation strategies in the detoxification of arsenic from polluted environments.

Abbreviations: AAS: Atomic Absorption Spectrophotometer, As: Arsenic, As (V): Sodium arsenate, As (III): sodium arsenite


Key words: Arsenic; As-resistance bacteria; Agricultural soil; 16S rRNA; Enterobacter asburiae; Enterobacter cloacae; aoxA and arsC genes


Selvi et al