Issues in Biological Sciences and Pharmaceutical Research
Vol.2(9),pp.090-105, October 2014
Article ID/14/BSPR/054/07 pages
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License
Original Research Article
Free radical-scavenging activity and cytotoxicity from leaves of Guettarda platypoda
Guilherme C. R. Rodrigues1, Isla V. G. A. Bastos1, Gibson G. Oliveira1, José A. S. Pereira-Junior1, Rômulo C. S. Peixoto Filho1, Macia Cleane S. Almeida2, Raimundo Braz-Filho3, Teresinha G. da Silva1, Telma L. G. Lemos2 and Sebastião J. de Melo1*
1 Department of Antibiotics, Federal University of Pernambuco (UFPE), Brazil.
2 Department of Organic and Inorganic Chemistry, Federal University of Ceará (UFC), Brazil.
3 Laboratory of Chemical Sciences, State University of North Fluminense Darcy Ribeiro, Brazil.
*Corresponding Author Email: melosebastiao(at)yahoo.com.br
The specie Guettarda platypoda DC. a shrub present in the flora of the state of Pernambuco (Itamaracá) is used in folk medicine as an antipyretic. The aim of this study was to conduct a bioactivity-guided fractionation of the leaves of this species, thus evaluating the biological activity (Cytotoxic and antioxidant) of substance and/or fractions of different polarities. The raw methanol extract of the leaves of G. platypoda was subjected to fractionation using solvents of increasing polarity; cell viability was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reduction assay; antioxidant activity was determined based on the ability to sequester free radicals by the method of DPPH (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and the molecular characterization was performed by spectrometric methods. From this work it was possible to isolate a porphyrin (pheophytin a). During bioguided tests, it was evident that the crude methanolic extract (IC50 = 7.02 x 10-3 mg/mL), fractions of ethyl acetate (IC50 = 5.58 x 10-3 mg/mL) and methanol (IC50 = 8.10-3 mg/mL) had higher antioxidant activity than vitamin C. In conclusion, the substances present in the extract in ethyl acetate (F3) of G. platypoda and in the F2.2 subfraction, demonstrated cytotoxic and antioxidant activity against all cell lines tested, but the pheophytin a (P5), first identified to the genus Guetarda, showed no significant activity against the tested cell lines in this work.
Key words: Guettarda platypoda, phytochemical, pheophytina, cytotoxic activity, antioxidant.