Issues in Biological Sciences and Pharmaceutical Research
Vol.Vol.6(3), pp. 30-38, December 2018
Available online at https://www.journalissues.org/IBSPR/
Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.
Original Research Article
Antifungal activity of silver nanoparticles synthesized using Citrus sinensis peel extract against fungal phytopathogens isolated from diseased tomato (Solanum lycopersicum L.)
Onyemaechi H. Obiazikwor1* and Hakeem O. Shittu1
1Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Benin, Benin City, Edo State, Nigeria.
*Correspondent Author Email:onyemaechi.obiazikwor(at)uniben.edu
Nanoparticles exhibit unusual physical, chemical and biological activity due to their reduced small sizes and they are applied in various disciplines including engineering, medicine and agriculture. The current study was carried out to investigate the antifungal activity of silver nanoparticles synthesized using Citrus sinensis peel extract against fungal phytopathogens isolated from diseased tomato. Silver nanoparticles were synthesized using Citrus sinensis peel extract and the formation of nanoparticles was monitored using spectrophotometry. The isolated fungal phytopathogens include Irenopsis, Diaporthe and Sphaerosporium spp. These phytopathogens were grown on potato dextrose agar supplemented with different concentrations of Citrus peel extract, silver nitrate solution or silver nanoparticles. Mycelia growth was recorded at 2 days’ interval for 8 days. From the results obtained, 100 % concentration of silver nanoparticles offered the highest antifungal activity with an inhibition rate of 82, 46 and 57% recorded for Irenopsis, Diaporthe and Sphaerosporium species, respectively after 8 days of culture initiation. The findings of this research indicated that silver nanoparticles were effective in controlling fungal isolates. Further studies should be carried out to investigate the mode of action and the use of silver nanoparticles in managing phytopathogens in crop plants, thereby ensuring food security.
Key words: Antimicrobial, crop plants, fungi, food security