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ZA Nermien
AA Fouad
AA Moustasfa
AH Dalia

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ZA Nermien
AA Fouad
AA Moustasfa
AH Dalia

Issues in Biological Sciences and Pharmaceutical Research
Vol.4(4),pp.17-34, July 2016
ISSN 2350-1588
Article ID /16/BSPR024/ 18 pages
Available online at
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.

Original Research Article

The preventive effect of grape seeds ethanolic extract as antihyperglycemia against streptozotocin-induced diabetic rats

1*Nermien Z. Ahmed, 2Fouad A. Ahmed, 2Moustasfa A. A. and 1Dalia A. Hashim

1National Organization for Drug Control & Research “NODCAR”; Department of Molecular Drug Evaluation, Giza, Egypt.
2Faculty of Agriculture, Cairo University, Department of Biochemistry, Giza, Egypt.

*Corresponding Author Email: nermienteleb(at)
Tel.: (020) 01223740700; 01069194681.

date Received: May 25, 2016     date Accepted: July 14, 2016     date Published: July 28, 2016


This study aim to determine the protective effect of Grape seeds (Vitis vinifera) ethanolic extract against streptocotozin-induced diabetic rats. Sterptozotocin was used by (50mg/kg b.w. i.p.); Vitis vinifera seeds were used at (100 & 200 mg/kg b.w. oral) daily/ three weeks.  The result indicated that Sterptozotocin increased the levels of serum glucose; uric acid; creatinine; ALP; ALT and AST, blood urea, and decreased the activities of SOD and GSH-Px, after treated with grape seeds by the high dose show a significantly decreased in levels of serum glucose; uric acid; creatinine, ALP; ALT and AST, urea. On the other hand, SOD and GSH-Px activities exerted a highly increase in diabetic rats given with the extract. Also, it can be speculated that Grape seed (Vitis vinifera) ethanolic extract has a hypoglycemic effect possibly related to the phytochemical compounds of Vitis vinifera plant seeds include Gallic acids, Catechin, Ρ-hydroxy bezoic acid, Vanillin, Ferulic acid, Ρ-Coumaric acid, Euganol , as well as, 5,7- dihydroxy 4-methoxy isoflavone which can optimize sugar uptake in the liver. These feats could induce insulin secretion and release from cells, as well as stimulates the tissue’s insulin sensitivity leading to an increase of the tissues’ glucose uptake, storage, and oxidation.

Key words: DPPH, Antioxidant, Lysosomal enzymes, Grape seeds (Vitis vinifera) extracts, Sterptozotocin

Nermien et al