Issues in Biological Sciences and Pharmaceutical Research
Vol.4(6),pp.58-64, September 2016
Article ID /16/BSPR026/07 pages
Available online at https://www.journalissues.org/IBSPR/
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License
Original Research Article
In vitro antioxidant activity of Oudneya africana R. Br. aerial parts
Larbi Zakaria Nabti1 and Rachid Belhattab2
1Departement of Natural Sciences, Higher School of Constantine, 25000 Constantine, Algeria.
2Departement of Biochemistry, Ferhat AbbasSétif-1 University, 19000 Setif, Algeria.
*Corresponding Author Email: nabti.zakaria(at)ensc.dz; nature_zak(at)hotmail.com
The aim of this study was to evaluate the antioxidant activity of three extracts; aqueous (Aq.E), hydro-alcoholic (MeOH/H2O.E) and methanol extract (Me.E ) obtained by using a Soxhlet apparatus, of the aerial parts of Oudneya africana a medicinal plant used in traditional pharmacopoeia of Algeria. The determination of total phenolic content by the Folin-Ciocalteu method produced values of: 108.6, 137.16 and 143.26 µg gallic acid equivalent/mg (GAE/mg) while the evaluation of total flavonoids content using Aluminum chloride (AlCl3) method showed that this plant contain 15.62, 21.96 and 27.63 µg quercetin equivalent/mg (QE/mg) for Aq.E, MeOH/H2O.E and Me.E, respectively. Several in vitro antioxidant methods such as DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging, iron ion chelation and ß-carotene/linoleic acid bleaching test have been assayed on the O. africana extracts. The three extracts showed a very interesting DPPH radical scavenging with low values of IC50 (12.05, 14.91 and 24.57 µg/ml for Me.E, Aq.E and MeOH/H2O.E, respectively), and a powerful ability to protect the oxidation of the ß-carotene/linoleic acid coupled with values of RAA (relative antioxidant activity) exceeded 80%. However, the iron ion chelation assay showed that the three extracts had the ability to complex ferrous (Fe2+) ions in a concentration-dependent manner. The different IC50 values obtained were 247.11 µg/ml for Aq.E, 826.12 µg/ml for MeOH/H2O.E and 2838.73 µg/ml for Me.E. These values are less interesting in comparaison with the standard EDTA value (IC50 = 9.79 µg/ml). The results obtained from the different assays showed that the aerial parts of O. africana could be exploited as a source of antioxidant compounds.
Key words: Oudneya africana, phenolics, flavonoids, radical scavenging, antioxidant