Issues in Biological Sciences and Pharmaceutical Research
Vol.2 (3), pp. 019-034,March 2014
Article ID BSPR005/14/ 06 pages
Copyright © 2014 Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 3.0 International License
Original Research Paper
Biodegradation of Griseofulvin by Bacillus subtilis isolated from expired pharmaceuticals raw materials
Accepted 27 February, 2014
*Maher Yahia Ahmed and Reda Ahmed Abd El-Mageed Bayoum
Department of Botany and Microbiology, Faculty of Science (Boys), Al-Azhar Univerisity, Cairo, Egypt.
*Corresponding Author’s Email: firstname.lastname@example.org
The enormous amount of the pollutants being dumped constantly from the pharmaceutical industries caused environment contamination. This pollutant can be removed by various technologies are more expensive; do not completely destroy rather transforming to desirable compounds. Biodegradation is used to eliminate the above contaminant completely from the environment with very low operation cost. Eleven bacterial isolates from six expired pharmaceutical raw materials on Tryptic Soy Agar (TSA) and Nutrient Agar (NA) medium from total different seventy-eight samples of expired pharmaceutical raw materials which collected from the storage room of Memphis Company for pharmaceutical and chemical industry, located in Cairo, Egypt. All bacterial isolates were identified by morphological, physiological and biochemical characteristics as according to Bergey’s Manual of Determinative Bacteriology. Studying the optimum conditions of Griseofulvin biodegrading enzyme was produced by Bacillus subtilis. The physical and chemical properties of biodegraded Griseofulvin were studied by using TLC, UV, IR, and HPLC analysis techniques. The enzyme responsible for the biodegradation process was precipitated by using saturated ammonium sulfate, and then concentrated using dialysis, purified using DEAE-Cellulose and sephadex G-200 column chromatography. The polyacrylamide gel electrophoresis was used for determining the Griseofulvin degrading enzyme purity, together with retention factor (Rf) and molecular masses (Mr). The biodegradation product of Griseofulvin is identified by using Gas Chromatography-Mass Spectrometry.
Key words: Griseofulvin, biodegradation, expired pharmaceutical raw materials, Bacillus subtilis