Issues in Biological Sciences and Pharmaceutical Research
Vol.2(6),pp. 049-053, July 2014
Article ID BSPR031,06 pages
Copyright © 2014 Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 3.0 International License
Original Research Paper
Histomorphological studies of the mother sporocyst of Schistosoma mansoni developed in tentacles of Biomphalaria glabrata snails
Accepted 23 June, 2014
I.B. Kalhoro*1, Safia Kalhoro2, Hameeda Kalhoro3 and Halima Kalhoro4
1Department of Anatomy and Histology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam,Pakistan.
2Department of Biotechnology and Genetics Engineering, University of Sindh Jamshoro, Pakistan.
3Department of Fresh Water Biology and Fisheries, University of Sindh Jamshoro, Pakistan.
4Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pakistan
*Corresponding Author Email:ibkalhoro(at)gmail.com
Susceptible and resistant snails Biomphalaria glabrata were infected with miracidia of Schistosoma mansoni from 1 to 10 days post-exposure. Histological serial sections revealed that throughout infection mother sporocysts were developed in tentacles of the susceptible, whereas, these were observed in resistant snails from 1-5, 7 and 9 days post-exposure at penetration point. Out of 50 susceptible snails, 22 animals had several mother sporocysts developed in the tentacles, whereas, in the resistant snails 10 animals had few mother sporocysts observed. There are 22 infected susceptible (n = 22) and 10 resistant snails (n = 10). Chi-square test shows highly significant differences between the number of infected tentacles of the susceptible and resistant snails (∑χ² (1) = 6.62, P< 0.01). In the susceptible snail single, multiple, mature and migratory types of mother sporocysts were 21, 77, 6 and 1, total (n = 105) and in the resistant snails single 10 and multiple 3 mother sporocysts (n = 15) were observed. Chi-square test shows highly significant differences between the number of the various types of the mother sporocysts found in the tentacles of the susceptible and resistant snails (∑χ²(3) = 17.31, P< 0.01). The healthy mother sporocysts is characterized by outer light thick tegument with cells, vacant spaces, muscles, anteriorly strongly stained penetration gland, neural mass in bottom containing PAS positive material, some germinal cells are scattered throughout body. The range means diameter of mother sporocysts in the susceptible snails 37.16 ± 4.04 mm to 75.24 ± 9.61 mm at 2 and 9 days post-exposure. Similarly, in the resistant snails the range mean diameter of the mother sporocysts was 34.22 ± 3.28 mm to 57.05 ± 5.88 mm at 2 and 9 days post-exposure. On the basis of above findings it is concluded that normal development and appearance of of mother sporocysts in the susceptible as compared to resistant snails during schistosome infections.
Key words: – Histomorphology, Tentacle mother sporocyst, Schistosoma mansoni, Susceptible and resistant snails.