International Research Journal of Public and Environmental Health
Vol.4 (1),pp. 11-21, January 2017
Available online at https://www.journalissues.org/IRJPEH/
Article 16/ID/JPRH090/ 11 pages
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.
Original Research Article
Protective effects of myrtle flavored bovine milk against ulcerative colitis in rats: Involvement of oxidative stress
Mohamed-Amine Jabri1,2*, Haifa Tounsi3, Afifa Abdellaoui3, Lamjed Marzouki2, Mohsen Sakly1 and Hichem Sebai1,2
1Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Université de Carthage, Tunisia ;
2Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Ressources – Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba – B.P. 382 – 9000 Béja, Tunisia ;
3Laboratoire d’anatomie pathologique humaine et expérimentale, Institut Pasteur de Tunis, 13, Place Pasteur, Tunis 1002, BP-74, Tunisia
* Corresponding Author E-mail : jabri.amino(at)gmail.com
Tel: +(216) 93 484 751,
Fax : +(216) 72 590 566
We aimed in the present to study to evaluate the protective effect of myrtle berries flavored bovine milk in acetic acid-induced ulcerative colitis (UC) model of inflammatory bowel disease in rats. Adult male Wistar rats were used and divided into five groups of ten each: control; UC; UC + bovine milk (BM) ; UC + myrtle flavored bovine milk (MFBM) (10 ml kg-1, b.w., p.o.); and UC + sulfasalazine (100 mg kg-1, b.w., p.o.). BM or MFBM (10 ml kg-1, b.w., p.o.) effectively suppressed the severity of colon injuries as evidenced by attenuation of histological morphological damages and colon weight/length ratio. BM or MFBM administration attenuated also the colon oxidative stress via reduction of malondialdehyde (MDA) level and restoration of non-enzymatic antioxidant such as thiols groups and reduced glutathione, antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx).and plasma scavenging activity (PSA). More importantly, BM or MFBM treatment also reversed all acetic acid induced-intracellular mediator perturbations. In conclusion, our data suggests that bovine milk flavored by myrtle berries exerted a potential protective effect against AA-induced colitis in rat owing in part to its antioxidant or by negatively regulating some intracellular mediators implicated in the Fenton reaction.
Key words: flavored bovine milk, colon, oxidative stress, thiols groups, functional beverage