Issues in Biological Sciences and Pharmaceutical Research
Vol.3 (5), pp.47-56, May 2015
Article ID /15/BSPR019/10 pages
Available online at https://www.journalissues.org/IBSPR/
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.
Original Research Article
Geophylogenetic analysis of Teinopalpus aureus Mell based on re-sequencing of the whole mitochondrial genome
Ying Huang1, Shanyi Zhou*1, Chaobin Huang1 and Juping Zeng2
1Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, 541004, China.
2College of Forestry, Jiangxi Agricultural University, Nanchang, 330045, China.
*Corresponding Author Email: syzhou(at)mailbox.gxnu.edu.cn
Tel: (+086)-773-5845952 (O), 18078337092 (Mobile)
The complete mitochondrial genomes of three geographic populations of Teinopalpus aureus Mell, i. e. the populations of Fujian Meihuashan (MHS), Jiangxi Jiulianshan (JLS), and Jiangxi Pingshan (PS), were sequenced and compared with that of the population of Guangxi Dayaoshan (DYS). The entire length of the T. aureus mitochondrial genome is among 15232–15237 bp. Sequence variation was found in different geographic populations and occurred in genes tRNAAla, tRNAGlu, ND5, 16SrRNA, and spacer regions. Among protein-coding genes, stop codons of ND5 gene were the same in all geographic populations. The same gene had the same start codon, but the stop codons sometimes varied among different geographic populations. Genetic relationships among them were analyzed. Sequences of a close species Papilio machaon from Papilionidae and a distant species Antheraea pernyi from Saturniidae downloaded from GenBank were used as outgroups. Maximum parsimony trees were constructed using 12S rRNA gene, COI gene, Cytb gene, and ND3 gene, respectively. Two lineages were found among the four geographic populations. One lineage was clustered by DYS and JLS, and the other lineage by MHS and PS. Relationship was close between DYS and JLS, and between MHS and PS.
Key words: Teinopalpus aureus, geographic populations, mitochondrial genome, genetic relationship