International Journal of Agricultural Policy and Research
Vol.5 (11), pp. 178-185, November 2017
Available online at https://www.journalissues.org/IJAPR/
Article 17/ID/JPR054/08/ pages
Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.
Original Research Article
Effect of extenders and storage periods on motility and fertilization rate of Silver Barb, Barbonymus gonionotus (Bleeker, 1850) semen
Balayet Hossen1, Md. Shakhawate Hossain1, Ibrahim Rashid*1, Md. Nahid Hasan1, Rokhsana Ethin1, Himal Thapa1 and Bhaskar Chandra Majumdar2
1Department of Fisheries Biology and Aquatic Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh.
2Department of Fisheries Technology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh
*Corresponding Author E-mail: ibrahim(at)bsmrau.edu.bd
The effects of short-term storage on the motility and fertilization rate of Barbonymus gonionotus semen were evaluated at different storage period. The best quality semen in terms of spermatological parameters were diluted with 5 different extenders viz. skim milk with fish ringer, glucose based extender, Alsever’s solution, egg yolk citrate and urea egg yolk at a ratio of 1:4, 1:6 and 1:9. Glucose based extender and skim milk with fish ringer solution at 1:4 and Alsever’s solution at 1:9 dilution ratios showed significantly the best equilibrium sperm motility (>80%). Similarly, the highest motility was obtained from semen stored for 30h with glucose based extender in portable ice box with ice at ~2°C. In fertilization trials, Alsever’s solution, glucose based extender and skim milk with fish ringer solution presented 5.85, 33.66 and 9.03% fertilization rate respectively after 24h of storage period compared to fresh sperm. Therefore, it can be concluded that glucose based extender is the best extender than the other extenders used in this study for the short term preservation of silver barb semen.
Key words: Barbonymus gonionotus, short-term preservation, cryo-diluent, equilibrium and post-thaw motility.