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LN Frison
S Sobrero
V Fernandez
MLZ Basílico

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LN Frison
S Sobrero
V Fernandez
MLZ Basílico

International Research Journal of Public and Environmental Health
Vol.2 (6),pp. 65-69, June 2015
ISSN 2360-8803
Available online at
Article 15/ID/JPRH037/05 pages
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.

Original Research Article

Susceptibility of black aspergili conidia to industrial sanitizers

Frison Laura N.1*, Sobrero Silvina2 ,Fernandez Verónica2 , and Basílico María L.Z1

1Laboratory of Microbiology, Department Food Engineering, Faculty of Chemical Engineering,Argentina.
2 FBCB Universidad Nacional del Litoral. (3000). Santa Fe, Argentina.

*Corresponding Author E-mail : lfrison(at)
Tel.: 54-342-4571164 (int. 2541); Fax: 54-342-4571162.

date Received:     date Accepted: May 25, 2015     date Published:


We compared the susceptibility of isolates of black aspergilli (Aspergillus section Nigri) conidia against sodium hypochlorite and peracetic acid. The suspension – neutralization UNE-EN1275 test was performed. Solutions of peracetic acid of 750, 1500, 2250, 3000 and 6000 mg/L and solutions of sodium hypochlorite of 100, 700, 1000 and 1300 mg/L were assessed. Cell suspensions were exposed to sanitizers for 1, 5, 15 and 30 minutes at 20 °C. Mean values of the inactivation kinetic data were fitted to a biphasic inactivation model with the GInaFiT macro software. The time for a 4-log10 reduction in conidia (4Dvalues) for sodium hypochlorite (700, 1000 and 1300 mg/L, pH 6 and 9) ranged from 1.1 to 28.8 minutes and for peracetic acid (6000 mg/L) ranged from 6.2 to 14.3 minutes. The results from this study demonstrate the need to identify contaminating flora because the response of molds pigmented and non-pigmented molds could be different.

Key words: Black aspergilli (Aspergillus section Nigri), industrial sanitizers, GInaFiT, peracetic acid, sodium hypochlorite, molds pigmented, sanitation processes

Frison et al