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HA Fernández
M Vittone
A Salomone
E y Manca

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HA Fernández
M Vittone
A Salomone
E y Manca

Issues in Biological Sciences and Pharmaceutical Research
Vol.3(6),pp.57-62,June 2015
ISSN 2350-1588
Article ID /15/BSPR018/06 pages
Available online at https://www.journalissues.org/IBSPR/
DOI:http://dx.doi.org/10.15739/ibspr.013
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License



Original Research Article

Biological silage of Merluccius hubbsi. Amino acid composition, degree of hydrolysis and peptides size

Fernández Herrero A., Vittone M. , Salomone A. and  y Manca E.

National Institute for Fisheries Research and Development, INIDEP.Paseo Victoria Ocampo N°1, 7600, Mar del Plata, Argentina.

*Corresponding Author E-mail : aherrero@inidep.edu.ar
Tel.: +54 223 486 2586



date Received: May 5, 2015     date Accepted: May 30, 2015     date Published: June 8, 2015


 Abstract

Aiming to achieve comprehensive utilization of marine resources through less polluting and more economical processes, we proceeded to the development of biological silage from waste Merluccius hubbsi. was used as inoculum yoghurth (10%); sucrose (10%) as a carbohydrate source and sorbic acid (0.25%) as antifungal. Was determined: moisture, protein, lipids and ash, as well as essential amino acids index (EAAI), degree of hydrolysis (DH) and the size of peptides to estimate the nutritional value of silage obtained by the fermentation of acid lactic. The EAAI, reveal the tryptophan as the limiting amino acid, followed by phenylalanine and methionine; while the other amino acids are present in sufficient quantities. The value of DH reached 42.34% in the 27th day, where proteins are prevalent among 49.1 kDa and 80 kDa, and smaller than 7.1 kDa. The results found in this study suggest that fish silage obtained by lactic fermentation, could be used as an alternative source of protein for animal feed because of its high nutritional value, chemical composition and degree of hydrolysis.


Key words: Merluccius hubbsi, biological silage, degree of hydrolysis, peptides.


Fernández et al