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 Abstract

Issues in Biological Sciences and Pharmaceutical Research Vol.1(1),pp.008-015,September 2013
Available online at https://www.journalissues.org/IBSPR/
© 2013 Journal Issues
Article ID BSPR07, 08 pages

Original Research Article

Determination of polyunsaturated fatty acids in Jatropha curcas somatic embryos  and the effect of abiotic sources

1*Demissie AG and 2Lele SS

1Department of Applied Biology,Faculty of Natural Science,Arba Minch University,Arba Minch, Ethiopia.
2Food Engineering and Technology Department,Institute of Chemical Technology (Deemed University)Matunga, Mumbai, 400 019, India

Corresponding Author Email: abebetse@yahoo.com
Tel.: 91-22-24145616
Fax: 91-22-24145614

Accepted 30 August, 2013

 Abstract

The present study reports the accumulation of polyunsaturated fatty acids and effect of media on Jatropha curcas somatic embryos. Plant cells were cultivated in three different growth media supplemented with various carbon, growth regulators and polyethylene glycol in triplicates. In addition The PUFA composition was determined using GC-MS and spectrophotometer. The maximum relative growth capacity of 0.621 and specific growth rate of 0.242 gm day-1 was found from cells cultivated in Murashige-Skoog media. The biomass productivity was found to be 4.531gmL-1 day-1. The fatty acid content of suspension culture and callus found to be 8+0.23% and 9+0.16% respectively extracted using chloroform: methanol (2:1). The GC-MS method for rapid determination of fatty acid profile is reliable and reproducible for further bioprocess and biotechnological studies of Jatropha tissue culture system.

Key words: Fatty acids, Jatropha curcas L., lipids, somatic embryos, suspension culture, callus


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Demissie and Lele