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D Chahra
M Ramdani
T Lograda
P Chalard
G Figueredo

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D Chahra
M Ramdani
T Lograda
P Chalard
G Figueredo

Issues in Biological Sciences and Pharmaceutical Research
Vol.4(5),pp.35-42, August 2016
ISSN 2350-1588
Article ID /16/BSPR028/08 pages
Available online at
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License

Original Research Article

Chemical composition and antimicrobial activity of Evernia prunastri and Ramalina farinacea from Algeria

Douibi Chahra1, Messaoud Ramdani1*, Takia Lograda1, Pierre Chalard2, 3, and Gilles Figueredo4

1Laboratory of Natural Resource Valorisation, SNV Faculty, Ferhat Abbas University Setif-1, 19000 Setif, Algeria.
2Clermont Université, ENSCCF, Institut de Chimie de Clermont-Ferrand, BP 10448, F-63000, France.
3CNRS, UMR 6296, ICCF, F-63171 Aubière, France.
4LEXVA Analytique, 460 rue du Montant, 63110 Beaumont, France.

*Corresponding Author Email: ramdanimessaoud(at)

Tel.: (213) 658101010
Fax: (213) 36937943

date Received: June 15, 2016     date Accepted: July 23, 2016     date Published: August 12, 2016


The chemical composition of essential oil, isolated from Evernia prunastri and Ramalina farinacea by hydrodistillation, was analysed by GC and GC/MS. A total 32 compounds representing 93.64% of the oil were identified in E. prunastri. This oil is characterised by an n-octadecanol (54.06%), n-tetradecanol (8.27%), Heptadecane (4.70%), Eicosene-1 (3.82%), n-hexadecanol (2.32%), Tridecenol acetate (2.05%). Ramalina farinacea with 34 compounds, representing 76.04% of the total oil, is characterised by the Manool (14.21%), n-octadecanol (10.24%), Eicosene-1 (8.26%), n-tetradecanol (7.68%), Manool oxide-13-epi (5.40%), α-pinene (4.04%), Abietal (3.75%). To test the antibacterial activity of essential oil of these species, four bacteria are used in this study. The oils have shown little effect against gram-negative bacteria, and modest antibacterial activity against Gram positive bacteria.

Key words: Lichens, Evernia prunastri, Ramalina farinacea, chemical composition, antibacterial activity, Babors, Algeria

Chahra et al