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K Boutemak
N Benali
N Moulai-Mostefa

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K Boutemak
N Benali
N Moulai-Mostefa

International Research Journal of Public and Environmental Health
Vol.4 (10),pp. 259-269, November 2017
ISSN 2360-8803
Available online at https://www.journalissues.org/IRJPEH/
DOI:https://doi.org/10.15739/irjpeh.17.030
Article 17/ID/JPRH009/ 011 pages
Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.



Original Research Article

Optimization of combination of steam distillation and solvent extraction of Artemisia campestris essential oil using RSM

Khalida Boutemak*1, Nasssima Benali2 and Nadji Moulai-Mostefa3

1LAFPC, University Saad Dahlab Blida1, Route de Soumaa, 09000 Blida, Algeria.
2Chemistry Department, University Saad Dahlab Blida1, Route de Soumaa, 09000 Blida, Algeria.
3LME, University of Medea, Ain D’Heb, 26001 Medea, Algeria.

*Corresponding Author E-mail : kboutemak(at)yahoo.fr



date Received: March 8, 2017     date Accepted: April 30, 2017     date Published: November 17, 2017


 Abstract

Extraction of essential oil (EO) from Artemisia campestris L. leaves using a combination of solvent extraction and steam distillation was investigated. A response surface methodology (RSM), in particular a Box-Behnken design was employed for the optimization of three parameters such as extraction time, liquid-solid ratio and extraction number for the achievement of high yield of extraction by solvent. The optimum extraction conditions were: extraction time of 2h, extraction number equal to 4 and ratio of liquid/solid of 6.5/1 (mL/g). Using these optimal parameters values, the mean obtained value of yield was 26.74 ± 0.11%. EO obtained by the combination of solvent extraction (SE) and steam distillation (SD) yielded 1.3% where the essential oil obtained by only steam distillation (SD) yielded 0.88%. Antibacterial activity showed that essential oil possessed a high activity and significant inhibitory power against Escherichia coli. EO isolated by SE-SD showed reducing ability and exhibited antioxidant activity; it had the best reducing power compared to the essential oil isolated by SD.


Key words: Artemisia campestris essential oil, extraction, steam distillation, optimization, RSM.


Boutemak et al