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J Bonou
H Ahouandjinou
F Baba-Moussa
Z Adéoti
V Dougnon
I Metongnon
JD Gbenou
F Toukourou
L Baba-Moussa

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J Bonou
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Issues in Biological Sciences and Pharmaceutical Research
Vol.4(6),pp.43-49, September 2016
ISSN 2350-1588
Article ID /16/BSPR032/07 pages
Available online at https://www.journalissues.org/IBSPR/
DOI:http://dx.doi.org/10.15739/ibspr.16.006
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.



Original Research Article

Assessment of the antimicrobial activity of essential oils from some Beninese medicinal plants:Influence of different tweens

J. Bonou1,2, H. Ahouandjinou1,2, F. Baba-Moussa1,2, Z. Adéoti2, V. Dougnon3, I. Metongnon1,2, J. D. Gbenou4, F. Toukourou2 and L. Baba-Moussa5*

1National Laboratory of the Quality Control of Medicines and Consumable Drugs known as ‘’ (LNCQ)’’, Ministry of Health, 06 BP 139 Cotonou, BENIN.
2Laboratory of Microbiology and Food Technology, Faculty of Science and Technology, University of Abomey-Calavi, ISBA-Champ de Foire, 01 BP 526 Cotonou, BENIN.
3Research Laboratory in Applied Biology known as ‘’(LARBA)’’, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 BP 2009 Cotonou, BENIN.
4Laboratory of Pharmacognosy and Essential Oils, Faculty of Health Sciences-Faculty of Science and Technology, University of Abomey-Calavi, ISBA-Champ de foire, Cotonou, BENIN.
5Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Technology, University of
Abomey-Calavi, 05 BP 1604 Cotonou, BENIN.

* Corresponding Author Email:laminesaid@yahoo.fr
Tel: +229 97 12 34 68



date Received: August 2, 2016     date Accepted: September 26, 2016     date Published: September 5, 2016


 Abstract

Medicinal plants have an important place in the therapy mechanism of Benin’s population. According to the World Health Organization, 75% of African populations use plants for treatment. Many researchers used to work with tweens. Thus, this work aimed to study the influence of the use of different types of tween in the evaluation of the antimicrobial activity of four essential oils. It helped also to determine the minimum inhibitory and bactericidal concentrations of those oils including their chemical compositions. Essential oils from some plants were obtained. The sensitivity of germs namely Micrococcus luteus ; Staphylococcus aureus ATCC 29213 ; Proteus mirabilis ATCC 24974 ; Pseudomonas aeruginosa ATCC 27853 ; Candida albicans IP 4872 to the various essential oils by microdilution technique was tested. Two chromatographic analyzes were performed for each oil on a gas chromatograph electronically controlled pressure. Results and Implications: Tween 60 has the best features, tweens 20 and 40 at 5% do not allow having a good dispersion of the essential oils in liquid media and a good spread in agar media. Furthermore, tween 80 seems to have a synergistic action with the essential oils although it shows good results. The chemical analysis showed several major compounds: myrcene (11.48%), neral (33.53%), geranial (43.10%), estragole (97.10%), limonene (61.40%), 1,8-cineole (61.60%). The minimum inhibitory concentrations (MIC) of the oils range between 0.078 and 10 mg / ml and the Minimum Bactericidal Concentrations (CMB) range from 0.078 to 10 ml /ml.


Key words: Essential oil, Tween, medicinal plants, antimicrobial activity, mouthwash.


Bonou et al