Issues in Biological Sciences and Pharmaceutical Research
Vol.8 (1),pp.1-19, February 2020
Available online at https://www.journalissues.org/IBSPR/
Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.
Original Research Article
Thymoquinone and Bronchodilation: The possible mechanism and therapeutic potential of an emerging natural drug in reactive airway disease
Adnan Bashir1, Yasir Arfat2*, Madiha Rasheed3, Salman Iftikhar4,Rao Salman Aziz4,Maheen Rana5 and Muhammad Rashid6
1Department of Pharmacology, Fatima Memorial College of Medicine and Dentistry Lahore, Pakistan
2King College of Bioresources Chemical and Material Engineering, Shaanxi University of Science and Technology, Xian, China.
3Department of Oral Biology, Wateem Dental College Rawalpindi, Pakistan
4Department of Pharmacology, Rashid Latif Medical and Dental College Lahore, Pakistan.
5Department of Pathology, Rashid Latif Medical and Dental College Lahore, Pakistan.
6Faculty of Fisheries and Wildlife, University of Veterinary and Animal Sciences, Lahore, Pakistan.
*Corresponding Author Email: yasir(at)mail.nwpu.edu.cn
Thymoquinone has a quantitative relaxant effect on the tracheal smooth muscle. In this study, we also attempted to investigate the probable mechanism of action of thymoquinone by predisposing the tracheal tissue of our experimental model to carbachol, a muscarinic agonist that produces contractions in the tissue and propranolol which blocks β receptors. The study was carried out on tracheal tissue from two groups of guinea pigs. Group 1 served as normal, while group 2 was sensitized and stimulated with ovalbumin to create airway hyper-responsiveness. We compared the percentage of relaxation produced by thymoquinone and salbutamol. It was observed that in the presence of high concentrations of the agonist, thymoquinone produced more relaxation in both sets of the experiment. It caused relaxation in the presence of propranolol, but it was not completely reversed. The relaxation produced by thymoquinone was also compared to salbutamol (conventionally used Beta agonist in reactive airway disease) and was observed to be less. Our results suggest that thymoquinone has properties of the muscarinic blockade and, among other mechanisms involved in the relaxation of smooth muscles relaxation, also has β2 agonistic activity.
Key words: Thymoquinone, ovalbumin, salbutamol, carbachol, propranolol, reactive airway disease.