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MJ Bangou
KB Koama
TH Coulidiati
RN-T Meda
AME Thiombiano
M Traoré-Coulibaly
OG Nacoulma

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MJ Bangou
KB Koama
TH Coulidiati
RN-T Meda
AME Thiombiano
M Traoré-Coulibaly
OG Nacoulma

Issues in Biological Sciences and Pharmaceutical Research
Vol.7(3),pp.46-57, September 2019
ISSN 2350-1588
Available online at https://www.journalissues.org/IBSPR/
DOI:https://doi.org/10.15739/ibspr.19.006
Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.



Original Research Article

Phytochemical screening, antioxidant and in vivo antiplasmodial activities of Acacia gourmaensis A. RICH. (Mimosaceae)

Bangou M.J.1,2*, Koama K.B.3, Coulidiati T.H.2,4, Meda R.N-T.1,2 Thiombiano A.M.E.2, Traoré-Coulibaly M.3,5 and Nacoulma O.G.2

1Unité de Formation et de Recherche en Sciences et Techniques. Département de Biochimie-Microbiologie, Université Nazi BONI, 01 BP 1091 Bobo-Dsso 01, Burkina Faso.
2Laboratoire de Biochimie et de Chimie Appliquées (LABIOCA), Unité de Formation et de Recherche en Sciences et Techniques. Département de Biochimie-Microbiologie, Université Ouaga I, 09 BP 848 Ouagadougou 09, Burkina Faso.
3Direction Régionale de l’Institut de Recherche en Sciences de la Santé (IRSS-DRO/CNRST). BP 545 Bobo-Dioulasso, Burkina Faso.
4Unité de Formation et de Recherche en Sciences et Techniques. Université Norbert ZONGO, 01 BP 376 Koudougou 01, Burkina Faso.
5Centre Muraz, BP 390, Bobo-Dioulasso, Burkina Faso.

*Corresponding Author E-mail: feliciabangou(at)yahoo.fr

Tel.:+(226)70399381



date Received: July 31, 2019     date Accepted: September 6, 2019     date Published: September 20, 2019


 Abstract

In this present study, in vivo antiplasmodial and antioxidant activities of Acacia gourmaensis A. RICH. (Mimosaceae) were evaluated based on its total extracts (ethanol-water, decoction, acetone), fractions (hexane fraction, ethyl acetate fraction, butanol fraction and aqueous fraction) and mineral contents. Total extract of decoction, acetone and EtOH-H2O fractions were used to evaluate chemical composition using HPLC-GC/MS-DAD methods. This plant is used in traditional medicine against liver disease, fractures, coughing fits and especially paludism. The antioxidant property was analyzed using 3 methods such as total antioxidant activity (TAC), iron reducing power (IRP) and radical scavenging activity (DPPH*). Antiplasmodial activity was performed in mice using red blood infected by Plasmodium berghei. The biological activities (antioxidants and antiplasmodial) were directed against plant phytochemical content 9 polyphenolic compounds were highlighted in the HPLC-DAD method, against 3 in HPLC- GC/MS. EtOH-H2O gave the best anti-TAC with 6.53 ± 0.15 mgEAA/mL. However, this extract (EtOH-H2O) gave the weakest activity with radical scavenging activity (23.73 ± 0.11 µg/mL). The best anti-DPPH* is obtained with acetone extract (1.93 ± 0.05 µg/mL). Concerning anti-IRP activity, hexane fraction gave the best result with 0.12 ± 0.00 µg/mL. At the antiplasmodial level, the best percentage reduction (19.9 %) is obtained with a dose of 150 mg/kg. On the phytochemistry plan, aqueous and ethyl acetate fractions given the best content with respectively 119.14 ± 8.13 mg EAG/g for total phenolic and 5.33 ± 0.17 EQ/g for total flavonoids. Mineral content quantification show that this plant is rich in calcium with 23.43 ± 1.32 mmol/L. The results due to the antiplasmodial activity could justify the traditional use of this plant.


Key words: antiplasmodial, antioxidant, phytochemistry, Acacia gourmaensis


Bangou et al