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DN Anong
J Fru-Cho
KS Mbandi
NA Amambua
T Nkuo-Akenji
VPK Titanji

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DN Anong
J Fru-Cho
KS Mbandi
NA Amambua
T Nkuo-Akenji
VPK Titanji

Issues in Biological Sciences and Pharmaceutical Research
Vol.4(3),pp.11-16, April 2016
ISSN 2350-1588
Article ID /15/BSPR052/06 pages
Available online at https://www.journalissues.org/IBSPR/
DOI:http://dx.doi.org/10.15739/ibspr.16.003
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.



Original Research Article

Expression pattern of immunoglobulin G subclasses in response to UB05 antigen in a plasmodium falciparum endemic area in Cameroon

1*Anong DN, 1Fru-Cho J, 2Mbandi KS, 3Amambua NA, 1Nkuo-Akenji T and 1Titanji VPK

1Department of Microbiology and Parasitology. University of Buea, Cameroon
2The Institute of Infectious Diseases and Molecular Medicine, Cape Town, S. Africa
3Medical Research council, Banjul, The Gambia.

*Corresponding Author Email: damian.anong@ubuea.cm

Tel.: +237677734816



date Received: December 12, 2015     date Accepted: April 12, 2016     date Published: April 26, 2016


 Abstract

The aim of this study was to show that UB05 a newly identified malaria antigen elicits protective antibody immune responses mainly of IgG1 and IgG3 subclasses. The study population comprised 240 randomly selected adults and children, febrile and none febrile, grouped into various age groups who consulted at the Health Post in Bolifamba. Serum from each individual was analyzed by Enzyme linked immunosorbent assay (ELISA) for the presence of antibodies to UB05 of the IgG subclasses. The levels of all IgG subclasses to UB05 increased with age and were significantly higher in adults than in infants (P<0.05). Cytophilic antibodies, IgG1 and IgG3 were significantly higher in adults than in children (p<0.05). IgG3 reactivity was generally higher when compared with IgG1 levels for all age groups and the difference was statistically significant (p<0.0001). There was a negative correlation between IgG3 antibodies and parasite density.  Individuals with fever had lower levels of IgG3 when compared with those without fever and this was most obvious in children. IgG2 and IgG4 responses to UB05 were generally higher in infected   subjects although IgG4 responses were lowest across all age groups. We conclude that UB05 elicits   protective immunity against malaria.


Key words: UB05, malaria antigen, immune response, IgG subclasses


Anong et al