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EA Fasakin
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Issues in Biological Sciences and Pharmaceutical Research
Vol.7(3),pp.35-45, September 2019
ISSN 2350-1588
Available online at https://www.journalissues.org/IBSPR/
DOI:https://doi.org/10.15739/ibspr.19.005
Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.



Original Research Article

Bioactive constituents and antimicrobial activity of essential oil of ginger (Zingiber officinale) on microorganisms isolated from smoke-dried catfish (Clarias gariepinus)

1*Akise Ogheneughwe Godwin,2Fasakin Emmanuel Adedayo and 2Adeparusi Eunice Oluwayemisi

1Department of Fisheries and Aquaculture, Faculty of Marine Environmental Management, Nigeria Maritime University, Okerenkoko, Delta State, Nigeria.
2Department of Fisheries and Aquaculture Technology, School of Agriculture and Agricultural Technology, Federal University of Technology Akure, Ondo State, Nigeria.

*Corresponding Author Email: ogakise(at)yahoo.com.au

Tel.: +2348036100501



date Received: March 6, 2019     date Accepted: April 25, 2019     date Published: September 20, 2019


 Abstract

Essential oils are gaining prominence because of their antimicrobial and antioxidative properties. This study aims to investigate the bioactive constituents and antimicrobial activity of essential oil of Ginger (Z. officinale) on microorganisms isolated from smoke-dried Catfish (Clarias gariepinus). Dried Z. officinale rhizomes were obtained from Oba market in Edo State. Essential oil was extracted using steam distillation and the chemical composition of oil was determined using the gas chromatography-mass spectroscopy (GC-MS) technique. The antimicrobial activity Z. officinale was evaluated using agar well diffusion method. The results showed that Z. officinale contained thirty-three compounds which consisted of 4.94% monoterpene hydrocarbons, 58.45% sesquiterpene hydrocarbons, 5.28% oxygenated sesquiterpenes and 28.34% oxygenated monoterpenes. The most abundant compound were beta.-Sesquiphellandrene (16.53%), Cyclohexene,1-methyl-4-(5-methyl-1-methylene-4-hexenyl)-, (S)- (13.87%), alpha.-Curcumene (8.34%) and (-)-Zingiberene (8.02%). Z. officinale oil elicited antibacterial activity against both Gram positive and negative bacterial isolates. The essential oil had a higher antibacterial activity than antifungal activity. The MIC, MBC and MFC studies revealed that Z. officinale was biocidal at the minimal concentration of 150 mg/mL against five bacterialisolates; B. lichenformis DSM 13, P. aeruginosa AAU2, S. epidermidis PM221, S. aureus NCTC 8325, P. aeruginosa PB112 165 and three fungal isolates; Aspergillus flavus, Mucor sp. and Rhizopus sp.


Key words: Bioactive components, essential oils, antimicrobial activity, Zingiber officinale, well diffusion method, Clarias gariepinus.


Akise et al