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FI Akinnibosun
FC Nwafor

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FI Akinnibosun
FC Nwafor

International Research Journal of Public and Environmental Health
Vol.2 (4),pp. 49-55,April 2015
ISSN 2360-8803
Available online at
Article 15/ID/JPRH011/ 07 pages
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License

Original Research Article

Prevalence of diarrhoea and antibiotic susceptibility test in children below 5 years at University of Benin Teaching Hospital, Nigeria

*Akinnibosun F. I. and Nwafor F. C.

Department of Microbiology, Faculty of Life Sciences, University of Benin, P. M. B. 1154, Benin City, Edo State, Nigeria.

*Corresponding Author E-mail: fakinnibosun(at)
Tel.: +2348146247781

date Received:     date Accepted: March 26, 2015     date Published:


Diarhoea disease is among of leading public health significance among children of below five years where that more than 315,000 deaths of preschool age children are recorded annually in Nigeria. A survey to determine the significance of bacterial species as possible pathogenic microorganisms that cause diarrhoea was carried out in the University of Benin Teaching Hospital (UBTH), Benin City, Nigeria. The age, sex, prevalence and maternal level of education in relation to diarrhoea in children younger than five years of age were determined. A questionnaire was used for collecting information on socio-economic characteristics, environmental hygiene and behavioural practices of patients and their care givers. Faecal specimen from 50 children, less than 60 months (5 years) old, were collected and assessed for microbiological profile of enteric pathogens. Antimicrobial susceptibility tests were performed on all identified isolates using the Kirby-Bauer disc diffusion method. The prevalence of infectious diarrhoea was age specific being highest at the age of 7 – 12 months and lowest at the age of 37-48 months. Escherichia coli was the most frequently isolated bacteria in all age groups (62.58%). However, the isolation rate of Staphylococcus aureus and Klebsiella sp. in this study showed these bacteria as veritable aetiologic pathogens of infectious childhood diarrhoea. Antibiotics susceptibility test conducted revealed that the isolates were most resistant to doxycycline followed by ciprofloxacin while the isolates were most susceptible to amoxicillin. Proper education and awareness are keys to combat this public health problem caused by these pathogens.

Key words: Diarrhoea, prevalence, susceptibility, antibiotic disc, watery stool.

Akinnibosun and Nwafor