Issues in Biological Sciences and Pharmaceutical Research
Vol.6(2),pp.23-29, September 2018
Available online at https://www.journalissues.org/IBSPR/
Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.
Original Research Article
Efficacy of artemether-lumefantrine on malaria parasite and its effect on some lipid profile in mice infected with Plasmodium berghei
Akanbi OM1*, Bakare OS2,. Adejuyigbe A1, Owoloye A1, Jimoh, M.A1. and Abiodun DO1
1Department of Animal and Environmental Biology, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Ondo State, Nigeria.
2Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Ondo State, Nigeria.
*Corresponding Author E mail: olusegun.akanbi(at)aaua.edu.ng
Artemisinin Combination Therapy (ACT) was introduced by WHO to prevent drug resistance by the malaria parasite, unfortunately ACT treatment failures have been reported in some malaria endemic areas, hence this work studied the antimalarial activity of artemether-lumefantrine, and its effects on some lipid profile in serum and different organs of mice infected with Plasmodium berghei. Thirty five albino male mice were divided into seven groups with five mice in each group. Six groups were infected with Plasmodium berghei NK 65 intraperitoneally, the seventh group was not infected (normal control). Five of the infected groups were treated with 0.5 mg/kg, 1.0 mg/kg, 1.5 mg/kg, 2.0 mg/kg and 2.5 mg/kg artemether-lumefantrine per body weight, while the sixth group was not treated (negative control). The treatment was observed daily for four consecutive days through oral intubation. The animals were sacrificed on the 5th day from the commencement of the treatment and the whole blood was collected into EDTA bottle, while the organs were collected into plain bottle and later homogenized in ice cold normal saline. The parasitaemia was monitored for 4 days of the treatment. The effect of treatment on lipid profile was also determined in serum, liver, kidney and heart. The result showed that there was significant increase (P<0.05) in the parasitaemia counts in the negative control when compared with the groups treated with artemether-lumefantrine. The parasite clearance rate was highest in the group treated with 0.25 mg/kg (87%) and list in the group treated with 1.0 mg/kg (36%). The triglyceride level was significantly higher (P<0.05) in the negative control than in the group treated with 1.5 mg/kg, 2.0 mg/kg and 2.5 mg/kg, while HDL and Cholesterol levels were significantly reduced in the negative control than in the group treated with 1.5 mg/kg, 2.0 mg/kg and 2.5 mg/kg. This study concluded that the efficacy of artemether-lumenfatrine was dose related and has effect on lipid profile.
Key words: Artemether-lumefantrine, malaria parasite, combination therapy, lipid profile