Issues in Biological Sciences and Pharmaceutical Research
Vol.7 (4),pp. 64-70, October 2019
Available online at https://www.journalissues.org/IBSPR/
Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.
Original Research Article
Toxicity evaluation of spinosad on male wistar rats
Adebiyi, Opeymi. E., Fadina, Olubunmi.O., Fayinminnu, Olajumoke.O., Idowu-Agida, Oladayo.O., Oladigbolu, Abiola.A., Oladosu, Bolanle. O and Dixon, Henry.A.
1National Horticultural Research Institute,Jericho Ibadan
2Department of Crop Protection and Environmental Biology, University of Ibadan
*Corresponding Author Email: adebiyiope(at)gmail.com
This study was carried out at the animal experimental house, department of Animal Science, University of Ibadan. Twenty rats weighing between 150-165g were used for the experiment for a period of five weeks, laid out in a Completely Randomized Design (CRD) with five groups of 4 rats per treatment including control. The rats were fed with Spinosad preserved maize incorporated feed. The doses of 0.5mg/kg, 1mg/kg (recommended), 1.5 and 2mg/kg were administered once daily for 3 weeks. Toxicological effects were studied and were analysed using descriptive statistics at p≥0.05. The results showed decrease in body weights in rats in accordance to increase in doses. Gross pathological cyst and irregularities were observed in all the livers while the kidneys showed no significant changes to spinosad. Some haematological parameters such as packed cell volume (PCV), Haemoglobin count (Hgb), Red blood cell (RBC), White blood cell (WBC), revealed no significant differences except the monocytes. The serum biochemical tests such as Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Albumin (Alb) and Globulin (Gb) revealed significant differences in values especially among rats treated. Comparism between the recommended (1.00mg/kg) and various levels of overdose (1.5 and 2.00mg/kg) has significant differences. Spinosad as a formulated form “Tracers®” under the present experimental conditions seems to be toxic to liver at a particular dosage by varied changes in cyst observed and in the levels of the heamatology, biochemical parameters and histopathology changes as a result of different concentrations in the treated groups relative to the control group. The overdoses of spinosad were found out to negatively affect liver and impaired its function; hence negatively impact the health of the animals. Abuse, misuse or overdoses of spinosad (bio-insecticide) should be discouraged.
Key words: Bio-insecticide, histological, organs, spinosad, serum biochemistry, wistar rats