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AM Abdel-Hadi
M Awad
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M Abdelkader
M Abo-Kadoum

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AM Abdel-Hadi
M Awad
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M Abdelkader
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Issues in Biological Sciences and Pharmaceutical Research
Vol.3 (11),pp.115-122, November 2015
ISSN 2350-1588
Article ID /15/BSPR044/05 pages
Available online at https://www.journalissues.org/IBSPR/
DOI:http://dx.doi.org/10.15739/ibspr.021
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.



Original Research Article

Viridicatin and Dehydrocyclopeptine, two bioactive alkaloids isolated from marine derived fungus Penicillium aurantiogriseum AUMC 9759

Ahmed M. Abdel-Hadi*1,3,M. Awad2,3,N. Abo-Dahab3, A. El-Shanawany3, M. Abdelkader4 and M.  Abo-Kadoum3

1Medical laboratories Department, College of Applied Medical Sciences, Majmaah University, Kingdom of Saudi Arabia
2Department of Biology, Faculty of Science, Taif University, Kingdom of Saudi Arabia
3Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut Branch, Egypt
4Pharmacognosy Department, Faculty of pharmacy,  Sohag University, Egypt.

*Corresponding Author Email: a.abdelhadi(at)mu.edu.sa



date Received: October 12, 2015     date Accepted: October 25, 2015     date Published: November 14, 2015


 Abstract

Chemical investigation of the marine derived fungus Penicillium aurantiogriseum AUMC 9759 isolated from sea sediment of Mediterranean Sea (Alexandria) has led to isolation of two bioactive alkaloids, Viridicatin (1) and Dehydrocyclopeptine (2) along with sub faction B (mixture of two sterols). The compounds were isolated and purified by combined chromatographic procedures. The structures were established by 1H and 13C NMR experiments and HRESIMS data. This is the first report for dehydrocyclopeptine 1H and 13C NMR assignments. LC-MS of total fungal extract revealed the presence of cyclopeptine, dehydrocyclopeptine, viridicatin, terrestric acid, viridicatic acid, roquefortine c and 4-hydroy-3,6-Dimethyl-2H-pyran-2-one. The crude extract of the strain exhibited high activities against four bacterial and four fungal strains. Viridicatin (1) showed strong activity against Mycobacterium tuberculosis. Sub faction B showed significant cytotoxicity against two cell line; hepatic cellular carcinoma (HEPG2) and breast cancer (mcf7) with IC50% of 32.8774 µg/ml and 24.3284 µg/ml respectively.


Key words: Viridicatin, Dehydrocyclopeptine, cytotoxicity, antimicrobial, P. aurantiogriseum


Abdel-Hadi et al